Utilization of anhydrous aluminum phosphate as a sole source of phosphorous by a selected carrot cell line

Hiroyuki Koyama, Kunihiko Ojima, Tomoyuki Yamaya

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    Two variants of carrot (Daucus carota L. cv. MS Yonsun) cell lines, which had been selected with Al-phosphate as a sole source of phosphorous, were characterized on their mechanisms of phosphate-utilization from Al-phosphate. Both cell lines excreted citrate into the medium. The amount of citrate excretion was highly correlated with cell growth in the presence of Al-phosphate. There was about a 1 to 1 correlation between solublized-Al and excreted-citrate in the medium during cell growth. These results suggest that 1) the citrate could chelate with Al, at a 1 to 1 ratio, in Al-phosphate, 2) the citrate-chelated Al remains outside the cells, and 3) solublized phosphate from Al-phosphate is utilized for the growth of carrot cells. The characteristics of the selected cells were very stable, since the rate of citrate-excretion showed no change after subculturing 25 passages without Al-phosphate.

    Original languageEnglish
    Pages (from-to)173-177
    Number of pages5
    JournalPlant and Cell Physiology
    Volume31
    Issue number2
    Publication statusPublished - 1990 Dec 1

    Keywords

    • Al-phosphate
    • Aluminum
    • Carrot cell
    • Citrate excretion
    • Daucus carota
    • Stress selection

    ASJC Scopus subject areas

    • Physiology
    • Plant Science
    • Cell Biology

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