Using Partial Nitrification and Anammox to Remove Nitrogen from Low-Strength Wastewater by Co-immobilizing Biofilm inside a Moving Bed Bioreactor

Rong Chen, Yasuyuki Takemura, Yuan Liu, Jiayuan Ji, Satoshi Sakuma, Kengo Kubota, Haiyuan Ma, Yu You Li

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

The stable operation of partial nitrification and anammox (PNA) process is a challenge in nitrogen removal from extremely low-strength ammonia wastewater like sewage mainstream. A moving bed reactor with functional carriers (30% filling rate) was developed to treat a synthetic influent with 50 mg/L ammonia. The long-term operation results showed nitrogen removal efficiencies of 71.7 ± 9.1% have been stably obtained under a relatively short hydraulic retention time of 2 h. Microbial analysis revealed anammox bacteria and ammonium oxidizing bacteria (AOB) with 29.7% and 6.32% abundance were the two most dominant bacteria in the reactor. Carriers largely retained slow-growing anammox bacteria in their hollow space and established a sandwich-like biofilm structure of co-immobilization of anammox bacteria and AOB. The anammox activity was much higher in carrier biofilms than in suspended flocs, while, for the AOB activity, the situation was reversed. Correspondingly, a fluorescent in situ hybridization analysis illustrated the active cell fractions of anammox bacteria and AOB in carrier biofilms were 63.7% and 4.8%, and 2.7% and 61.4% in suspended flocs. Biofilm formation and dissolved oxygen control were deemed to be the two key factors affecting the optimal co-immobilization of anammox bacteria and AOB, which guaranteed the efficient PNA.

Original languageEnglish
Pages (from-to)1353-1361
Number of pages9
JournalACS Sustainable Chemistry and Engineering
Volume7
Issue number1
DOIs
Publication statusPublished - 2019 Jan 7

Keywords

  • AOB
  • Anammox
  • Biofilm
  • Co-immobilization
  • Low-strength wastewater

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Renewable Energy, Sustainability and the Environment

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