Using a customized DNA microarray for expression profiling of the estrogen-responsive genes to evaluate estrogen activity among natural estrogens and industrial chemicals

Shunichi Terasaka, Yukie Aita, Akio Inoue, Shinichi Hayashi, Michiko Nishigaki, Kazuhiko Aoyagi, Hiroki Sasaki, Yuko Wada-Kiyama, Yasuo Sakuma, Shuichi Akaba, Junko Tanaka, Hideko Sone, Junzo Yonemoto, Masao Tanji, Ryoiti Kiyama

Research output: Contribution to journalArticlepeer-review

87 Citations (Scopus)

Abstract

We developed a DNA microarray to evaluate the estrogen activity of natural estrogens and industrial chemicals. Using MCF-7 cells, we conducted a comprehensive analysis of estrogen-responsive genes among approximately 20,000 human genes. On the basis of reproducible and reliable responses of the genes to estrogen, we selected 172 genes to be used for developing a customized DNA microarray. Using this DNA microarray, we examined estrogen activity among natural estrogens (17β-estradiol, estriol, estrone, genistein), industrial chemicals (diethylstilbestrol, bisphenol A, nonylphenol, methoxychlor), and dioxin. We obtained results identical to those for other bioassays that are used for detecting estrogen activity. On the basis of statistical correlations analysis, these bioassays have shown more sensitivity for dioxin and methoxychlor.

Original languageEnglish
Pages (from-to)773-781
Number of pages9
JournalEnvironmental health perspectives
Volume112
Issue number7
DOIs
Publication statusPublished - 2004 May

Keywords

  • Endocrine disruptor
  • Estrogenicity
  • Expression profile
  • Microarray

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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