Usefulness of quantitative real-time polymerase chain reaction in following up patients with Epstein-Barr virus infection after liver transplantation

Takashi Orii, Nobuhiro Ohkohchi, Hiroyuki Kikuchi, Nozomi Koyamada, Seiji Chubachi, Susumu Satomi, Hiroshi Kimura, Yo Hoshino, Makoto Morita

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Background. Post-transplant lymphoproliferative disease (PTLD), which is mainly induced by Epstein-Barr virus (EBV) infection, is a cause of significant morbidity and mortality for patients undergoing liver transplantation, especially when it is detected at such an advanced stage as monoclonal malignant lymphoma. Methods. In this series, 6 of 22 liver transplant patients suffered from EBV infection. We tested quantitative DNA (Qt-DNA) by real-time polymerase chain reaction (PCR), qualitative DNA in plasma (Q1-pDNA) by PCR, and EBV-encoded mRNA 1 (EBER 1) by in situ hybridization to clarify which of them is a better marker for the early diagnosis and prediction of EBV-associated disorders. Results. Four had signs or symptoms of PTLD, but 2 did not develop individualized lymphoid lesions. In all patients, both Qt-DNA and EBER 1 exceeded the cut-off level of 102.5 copies/μg DNA and 0.002%, respectively, at the time of diagnosis. In 2 patients, when Qt-DNA had a poor decline, EBER 1, even if it seemed to decrease after antiviral therapy, increased again after a few months and the clinical symptoms recurred. In 2 patients, Qt-DNA and EBER 1 increased again after a few months of antiviral therapy, and Q1-pDNA remained positive, whereas, in 3 patients, no reaction of EBV could be detected once Q1-pDNA became negative, even after the cessation of therapy. Conclusions. These results suggest that real-time PCR for Qt-DNA was more sensitive to the real-time activity of EBV and that Q1-pDNA could indicate when to stop antiviral therapy.

Original languageEnglish
Pages (from-to)308-317
Number of pages10
JournalClinical Transplantation
Volume14
Issue number4I
DOIs
Publication statusPublished - 2000 Jan 1

Keywords

  • Epstein-barr virus
  • Liver transplantation
  • Polymerase chain reaction
  • Post-transplant lymphoproliferative disorder

ASJC Scopus subject areas

  • Transplantation

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