Use of the avidin-biotin system for immobilization of an enzyme on the electrode surface

Junichi Anzai, Tomonori Hoshi, Shouryu Lee, Tetsuo Osa

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Two different procedures were developed for immobilizing an enzyme on the electrode surface by using the strong complexation of avidin and the biotinylated enzyme. The first method is based on the adsorption of avidin or a complex of avidin and the biotinylated enzyme on the LB film-coated electrode. The second procedure involves the direct electro-deposition of avidin or the avidin-biotinylated enzume complex. Response characteristics of glucose sensors prepared by both techniques were discussed in terms of the enzyme loading on the electrode surface.

Original languageEnglish
Pages (from-to)73-75
Number of pages3
JournalSensors and Actuators: B. Chemical
Volume13
Issue number1-3
DOIs
Publication statusPublished - 1993 Jan 1

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry
  • Electrical and Electronic Engineering

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