TY - JOUR
T1 - Use of polymeric indicator for electrochemical DNA sensors
T2 - Poly(4-vinylpyridine) derivative bearing [Os(5,6-dimethyl-1,10-phenanthroline)2Cl]2+
AU - Liu, Aihua
AU - Anzai, Junichi
PY - 2004/5/15
Y1 - 2004/5/15
N2 - A poly(4-vinylpyridine) (PVP) derivative bearing redoxactive osmium complexes, PVP-[Os(5,6-dmphen)2Cl]2+ (5,6-dmphen = 5,6-dimethyl-1,10-phenanthroline), was employed as a hybridization indicator for electrochemical DNA sensors. PVP-[Os(5,6-dmphen)2Cl] 2+ exhibited ∼1000 times higher sensitivity than the corresponding monomeric analogue, [Os(5,6-dmphen)3]2+, in DNA determination due to polymeric effects. The detection limit of the present sensor was ∼0.5 amol. Another merit of the polymeric indicator is that the redox potential was found to be +360 mV (vs Ag/AgCl), which is significantly lower than that reported for the monomeric analogue (+672 mV). The polymeric indicator was applicable to the discrimination of single- and double-base-mismatched DNAs from fully matched target DNA. The polymeric indicator can be removed from the electrode surface by rinsing the electrode in a high-temperature buffer for 6 min, and thus, the polymeric indicator-based DNA sensor can be used repeatedly.
AB - A poly(4-vinylpyridine) (PVP) derivative bearing redoxactive osmium complexes, PVP-[Os(5,6-dmphen)2Cl]2+ (5,6-dmphen = 5,6-dimethyl-1,10-phenanthroline), was employed as a hybridization indicator for electrochemical DNA sensors. PVP-[Os(5,6-dmphen)2Cl] 2+ exhibited ∼1000 times higher sensitivity than the corresponding monomeric analogue, [Os(5,6-dmphen)3]2+, in DNA determination due to polymeric effects. The detection limit of the present sensor was ∼0.5 amol. Another merit of the polymeric indicator is that the redox potential was found to be +360 mV (vs Ag/AgCl), which is significantly lower than that reported for the monomeric analogue (+672 mV). The polymeric indicator was applicable to the discrimination of single- and double-base-mismatched DNAs from fully matched target DNA. The polymeric indicator can be removed from the electrode surface by rinsing the electrode in a high-temperature buffer for 6 min, and thus, the polymeric indicator-based DNA sensor can be used repeatedly.
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U2 - 10.1021/ac0303970
DO - 10.1021/ac0303970
M3 - Article
C2 - 15144212
AN - SCOPUS:2442651711
VL - 76
SP - 2975
EP - 2980
JO - Industrial And Engineering Chemistry Analytical Edition
JF - Industrial And Engineering Chemistry Analytical Edition
SN - 0003-2700
IS - 10
ER -