Use of native gels to measure protein binding to SSB

Jin Inoue, Tsutomu Mikawa

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

We describe a procedure to detect protein binding to SSB by polyacrylamide gel electrophoresis under non-denaturing conditions. As an example, we show the interaction of Thermus thermophilus (Tth) SSB with its cognate RecO protein. The interaction is detected as decay of the band corresponding to SSB by addition of RecO. We also demonstrate analysis of the RecO-RecR interaction as another example of this method.

Original languageEnglish
Title of host publicationSingle-Stranded DNA Binding Proteins
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages175-182
Number of pages8
ISBN (Print)9781627030311
DOIs
Publication statusPublished - 2012 Jan 1
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume922
ISSN (Print)1064-3745

Keywords

  • Electrophoresis
  • Mutational analysis
  • Native-PAGE
  • Protein-protein interaction
  • RecO
  • RecR
  • SSB-binding protein

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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