Use of native gels to measure protein binding to SSB.

Jin Inoue, Tsutomu Mikawa

Research output: Contribution to journalArticlepeer-review

Abstract

We describe a procedure to detect protein binding to SSB by polyacrylamide gel electrophoresis under non-denaturing conditions. As an example, we show the interaction of Thermus thermophilus (Tth) SSB with its cognate RecO protein. The interaction is detected as decay of the band corresponding to SSB by addition of RecO. We also demonstrate analysis of the RecO-RecR interaction as another example of this method.

Original languageEnglish
Pages (from-to)175-182
Number of pages8
JournalMethods in molecular biology (Clifton, N.J.)
Volume922
Publication statusPublished - 2012
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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