Use of abasic site-containing DNA strands for nucleobase recognition in water

Keitaro Yoshimoto, Seiichi Nishizawa, Masakazu Minagawa, Norio Teramae

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

Nucleobase recognition in water is successfully achieved by the use of an abasic site (AP site) as the molecular recognition field. We intentionally construct the AP site in DNA duplex so as to orient the AP site toward a target nucleobase and examine the complexation of 2-amino-7-methylnaphthyridine (AMND) with nucleobases at the AP site. AMND is found to selectively bind to cytosine (C) base with a 1:1 binding constant of >106 M-1, accompanied by remarkable quenching of its fluorescence. In addition to hydrogen bonding, a stacking interaction with nucleobases flanking the AP site seems responsible for the binding properties of AMND at the AP site. Possible use of AMND is also presented for selective and visible detection of a single-base alternation related to the cytosine base.

Original languageEnglish
Pages (from-to)8982-8983
Number of pages2
JournalJournal of the American Chemical Society
Volume125
Issue number30
DOIs
Publication statusPublished - 2003 Jul 30

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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