Use of a thermal lens microscope in integrated catecholamine determination on a microchip

Hussein M. Sorouraddin, Akihide Hibara, Takehiko Kitamori

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)

Abstract

A new method for determination for catecholamines (CA) utilizing microchip technology and a thermal lens microscope has been developed. Microchannels with a 250 nmxlOO iim cross section were used for mixing, reaction, and detection. Epinephrine (EP), nor-epinephrine (NE), dopamine (DA), and L-dopa (LD) were determined by using coloring oxidization to aminochromes by sodium metaperiodate. A thermal lens microscope (TLM) was used for detection of the product. The sensitivity of the system was comparable for the four CA and required only 15 s for mixing of sample and reagent. The calibration lines indicated excellent linearity for concentrations of 5-20 μg mL-1. The relative standard deviations for 10 μg mL-1 solution were 1.08, 2.18, 2.2, and 2.5% for EP, NE, DA, and LD, respectively. CA in pharmaceutical injections were also determined by use of the system and the results correlated very well with nominal values. Results obtained by use of the integrated system suggested there was a sufficient possibility to realize high-throughput medical diagnosis systems.

Original languageEnglish
Pages (from-to)91-96
Number of pages6
JournalAnalytical and Bioanalytical Chemistry
Volume371
Issue number2
DOIs
Publication statusPublished - 2001
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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