Use of a clinical MRI scanner for preclinical research on rats

Akihide Yamamoto, Hiroshi Sato, Jun Ichiro Enmi, Kenji Ishida, Takayuki Ose, Atsuomi Kimura, Hideaki Fujiwara, Hiroshi Watabe, Takuya Hayashi, Hidehiro Iida

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

This study evaluated the feasibility of imaging rat brains using a human whole-body 3-T magnetic resonance imaging (MRI) scanner with specially developed transmit-and-receive radiofrequency coils. The T1- and T 2-weighted images obtained showed reasonable contrast. Acquired contrast-free time-of-flight magnetic resonance angiography images clearly showed the cortical middle cerebral artery (MCA) branches, and interhemispheric differences could be observed. Dynamic susceptibility contrast MRI at 1.17 mm3 voxel resolution, performed three times following administration of gadolinium diethylenetriamine pentaacetic acid (Gd-DTPA, 0.1 mmol/kg), demonstrated that the arterial input function (AIF) can be obtained from the MCA region, yielding cerebral blood flow (CBF), cerebral blood volume, and mean transit time (MTT) maps. The hypothalamus (HT) to parietal cortex (Pt) CBF ratio was 45.11 ± 2.85%, and the MTT was 1.29 ± 0.40 s in the Pt region and 2.32 ± 0.17 s in the HT region. A single dose of Gd-DTPA enabled the assessment of AIF within MCA territory and of quantitative CBF in rats.

Original languageEnglish
Pages (from-to)13-21
Number of pages9
JournalRadiological Physics and Technology
Volume2
Issue number1
DOIs
Publication statusPublished - 2009 Jan 1
Externally publishedYes

Keywords

  • Dynamic susceptibility contrast (DSC)
  • Human whole-body 3-T MRI scanner
  • Preclinical research
  • Quantitative mapping
  • Rat brain
  • Single dose of Gd-DTPA

ASJC Scopus subject areas

  • Radiation
  • Physical Therapy, Sports Therapy and Rehabilitation
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Yamamoto, A., Sato, H., Enmi, J. I., Ishida, K., Ose, T., Kimura, A., Fujiwara, H., Watabe, H., Hayashi, T., & Iida, H. (2009). Use of a clinical MRI scanner for preclinical research on rats. Radiological Physics and Technology, 2(1), 13-21. https://doi.org/10.1007/s12194-008-0038-x