TY - JOUR
T1 - Use of a clinical MRI scanner for preclinical research on rats
AU - Yamamoto, Akihide
AU - Sato, Hiroshi
AU - Enmi, Jun Ichiro
AU - Ishida, Kenji
AU - Ose, Takayuki
AU - Kimura, Atsuomi
AU - Fujiwara, Hideaki
AU - Watabe, Hiroshi
AU - Hayashi, Takuya
AU - Iida, Hidehiro
N1 - Funding Information:
Acknowledgments The authors would like to express our appreciation to the reviewers, the editors and the editorial assistants of Radiological Physics and Technology for their invaluable advice on how to improve our manuscript. This study was supported by a grant for research on Advanced Medical Technology from the Ministry of Health, Labor and Welfare, Japan. We would like to thank the VPL released by AZE Ltd. (Tokyo, Japan) and the software library provided by the Oxford University Center for Functional MRI of the Brain. We are grateful to the staff at the National Cardiovascular Center for their invaluable contributions and efforts. Last but not least, we would like to express our thanks to Miss Atra Ardekani (a summer intern from McGill University in Montreal, Quebec, Canada).
PY - 2009/1
Y1 - 2009/1
N2 - This study evaluated the feasibility of imaging rat brains using a human whole-body 3-T magnetic resonance imaging (MRI) scanner with specially developed transmit-and-receive radiofrequency coils. The T1- and T 2-weighted images obtained showed reasonable contrast. Acquired contrast-free time-of-flight magnetic resonance angiography images clearly showed the cortical middle cerebral artery (MCA) branches, and interhemispheric differences could be observed. Dynamic susceptibility contrast MRI at 1.17 mm3 voxel resolution, performed three times following administration of gadolinium diethylenetriamine pentaacetic acid (Gd-DTPA, 0.1 mmol/kg), demonstrated that the arterial input function (AIF) can be obtained from the MCA region, yielding cerebral blood flow (CBF), cerebral blood volume, and mean transit time (MTT) maps. The hypothalamus (HT) to parietal cortex (Pt) CBF ratio was 45.11 ± 2.85%, and the MTT was 1.29 ± 0.40 s in the Pt region and 2.32 ± 0.17 s in the HT region. A single dose of Gd-DTPA enabled the assessment of AIF within MCA territory and of quantitative CBF in rats.
AB - This study evaluated the feasibility of imaging rat brains using a human whole-body 3-T magnetic resonance imaging (MRI) scanner with specially developed transmit-and-receive radiofrequency coils. The T1- and T 2-weighted images obtained showed reasonable contrast. Acquired contrast-free time-of-flight magnetic resonance angiography images clearly showed the cortical middle cerebral artery (MCA) branches, and interhemispheric differences could be observed. Dynamic susceptibility contrast MRI at 1.17 mm3 voxel resolution, performed three times following administration of gadolinium diethylenetriamine pentaacetic acid (Gd-DTPA, 0.1 mmol/kg), demonstrated that the arterial input function (AIF) can be obtained from the MCA region, yielding cerebral blood flow (CBF), cerebral blood volume, and mean transit time (MTT) maps. The hypothalamus (HT) to parietal cortex (Pt) CBF ratio was 45.11 ± 2.85%, and the MTT was 1.29 ± 0.40 s in the Pt region and 2.32 ± 0.17 s in the HT region. A single dose of Gd-DTPA enabled the assessment of AIF within MCA territory and of quantitative CBF in rats.
KW - Dynamic susceptibility contrast (DSC)
KW - Human whole-body 3-T MRI scanner
KW - Preclinical research
KW - Quantitative mapping
KW - Rat brain
KW - Single dose of Gd-DTPA
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U2 - 10.1007/s12194-008-0038-x
DO - 10.1007/s12194-008-0038-x
M3 - Article
C2 - 20821124
AN - SCOPUS:64249123050
VL - 2
SP - 13
EP - 21
JO - Radiological Physics and Technology
JF - Radiological Physics and Technology
SN - 1865-0333
IS - 1
ER -
