Urocortin in human placenta and maternal plasma

Fumie Watanabe, Yutaka Oki, Megumi Ozawa, Masahiro Masuzawa, Masayasu Iwabuchi, Teruya Yoshimi, Tomizo Nishiguchi, Kazumi Iino, Hironobu Sasano

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

Plasma immunoreactive (IR-) urocortin (Ucn) and corticotropin-releasing factor (CRF) levels in pregnant women were measured by their specific radioimmunoassays after extraction. Although plasma IR-CRF levels were increased in pregnant women as compared to men and non-pregnant women, there was no difference of plasma IR-Ucn levels among groups. Ucn mRNA was detected in cytotrophoblasts and syncytiotrophoblasts by in situ hybridization. A reverse-phase high-performance liquid chromatography (HPLC) showed the major peak of IR-Ucn in placenta and plasma that had similar chromatographic mobility to synthetic Ucn1-40. These data suggest that Ucn is produced and processed into the same form of synthetic Ucn in placenta, but not secreted into maternal blood.

Original languageEnglish
Pages (from-to)205-209
Number of pages5
JournalPeptides
Volume20
Issue number2
DOIs
Publication statusPublished - 1999 Feb

Keywords

  • Corticotropin-releasing factor
  • MRNA
  • Placenta
  • Pregnancy
  • Urocortin

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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