Urocortin in human placenta and maternal plasma

Fumie Watanabe; Yutaka Oki; Megumi Ozawa; Masahiro Masuzawa; Masayasu Iwabuchi; Teruya Yoshimi; Tomizo Nishiguchi; Kazumi Iino; Hironobu Sasano

doi:10.1016/S0196-9781(98)00175-2

Urocortin in human placenta and maternal plasma

Fumie Watanabe, Yutaka Oki, Megumi Ozawa, Masahiro Masuzawa, Masayasu Iwabuchi, Teruya Yoshimi, Tomizo Nishiguchi, Kazumi Iino, Hironobu Sasano

MED - Medical Sciences

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

Plasma immunoreactive (IR-) urocortin (Ucn) and corticotropin-releasing factor (CRF) levels in pregnant women were measured by their specific radioimmunoassays after extraction. Although plasma IR-CRF levels were increased in pregnant women as compared to men and non-pregnant women, there was no difference of plasma IR-Ucn levels among groups. Ucn mRNA was detected in cytotrophoblasts and syncytiotrophoblasts by in situ hybridization. A reverse-phase high-performance liquid chromatography (HPLC) showed the major peak of IR-Ucn in placenta and plasma that had similar chromatographic mobility to synthetic Ucn1-40. These data suggest that Ucn is produced and processed into the same form of synthetic Ucn in placenta, but not secreted into maternal blood.

Original language	English
Pages (from-to)	205-209
Number of pages	5
Journal	Peptides
Volume	20
Issue number	2
DOIs	https://doi.org/10.1016/S0196-9781(98)00175-2
Publication status	Published - 1999 Feb

Keywords

Corticotropin-releasing factor
MRNA
Placenta
Pregnancy
Urocortin

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

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10.1016/S0196-9781(98)00175-2

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title = "Urocortin in human placenta and maternal plasma",

abstract = "Plasma immunoreactive (IR-) urocortin (Ucn) and corticotropin-releasing factor (CRF) levels in pregnant women were measured by their specific radioimmunoassays after extraction. Although plasma IR-CRF levels were increased in pregnant women as compared to men and non-pregnant women, there was no difference of plasma IR-Ucn levels among groups. Ucn mRNA was detected in cytotrophoblasts and syncytiotrophoblasts by in situ hybridization. A reverse-phase high-performance liquid chromatography (HPLC) showed the major peak of IR-Ucn in placenta and plasma that had similar chromatographic mobility to synthetic Ucn1-40. These data suggest that Ucn is produced and processed into the same form of synthetic Ucn in placenta, but not secreted into maternal blood.",

keywords = "Corticotropin-releasing factor, MRNA, Placenta, Pregnancy, Urocortin",

author = "Fumie Watanabe and Yutaka Oki and Megumi Ozawa and Masahiro Masuzawa and Masayasu Iwabuchi and Teruya Yoshimi and Tomizo Nishiguchi and Kazumi Iino and Hironobu Sasano",

note = "Funding Information: We thank Dr. Toshiro Fujii and Mr. Noriyuki Ito for their valuable help. This work is supported by Smoking Research Foundation. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",

year = "1999",

month = feb,

doi = "10.1016/S0196-9781(98)00175-2",

language = "English",

volume = "20",

pages = "205--209",

journal = "Peptides",

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T1 - Urocortin in human placenta and maternal plasma

AU - Watanabe, Fumie

AU - Oki, Yutaka

AU - Ozawa, Megumi

AU - Masuzawa, Masahiro

AU - Iwabuchi, Masayasu

AU - Yoshimi, Teruya

AU - Nishiguchi, Tomizo

AU - Iino, Kazumi

AU - Sasano, Hironobu

N1 - Funding Information: We thank Dr. Toshiro Fujii and Mr. Noriyuki Ito for their valuable help. This work is supported by Smoking Research Foundation. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.

PY - 1999/2

Y1 - 1999/2

N2 - Plasma immunoreactive (IR-) urocortin (Ucn) and corticotropin-releasing factor (CRF) levels in pregnant women were measured by their specific radioimmunoassays after extraction. Although plasma IR-CRF levels were increased in pregnant women as compared to men and non-pregnant women, there was no difference of plasma IR-Ucn levels among groups. Ucn mRNA was detected in cytotrophoblasts and syncytiotrophoblasts by in situ hybridization. A reverse-phase high-performance liquid chromatography (HPLC) showed the major peak of IR-Ucn in placenta and plasma that had similar chromatographic mobility to synthetic Ucn1-40. These data suggest that Ucn is produced and processed into the same form of synthetic Ucn in placenta, but not secreted into maternal blood.

AB - Plasma immunoreactive (IR-) urocortin (Ucn) and corticotropin-releasing factor (CRF) levels in pregnant women were measured by their specific radioimmunoassays after extraction. Although plasma IR-CRF levels were increased in pregnant women as compared to men and non-pregnant women, there was no difference of plasma IR-Ucn levels among groups. Ucn mRNA was detected in cytotrophoblasts and syncytiotrophoblasts by in situ hybridization. A reverse-phase high-performance liquid chromatography (HPLC) showed the major peak of IR-Ucn in placenta and plasma that had similar chromatographic mobility to synthetic Ucn1-40. These data suggest that Ucn is produced and processed into the same form of synthetic Ucn in placenta, but not secreted into maternal blood.

KW - Corticotropin-releasing factor

KW - MRNA

KW - Placenta

KW - Pregnancy

KW - Urocortin

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Urocortin in human placenta and maternal plasma

Abstract

Keywords

ASJC Scopus subject areas

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