Abstract
A potentiometric method for the determination of urea in human blood with an ion-sensitive field effect transistor coated with a urease-albumin membrane is described. The urea sensor exhibits a potentiometric response to urea concentrations in the range of 0.1—10 mm with a response time of ca. 3 min. Urea concentrations of diluted serum and plasma can be determined accurately with the sensor. Comparison with an official method (i.e. the urease-indophenol method) gave satisfactory results.
| Original language | English |
|---|---|
| Pages (from-to) | 693-698 |
| Number of pages | 6 |
| Journal | Chemical and Pharmaceutical Bulletin |
| Volume | 35 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1987 |
Keywords
- enzyme sensor
- human blood
- ion-sensitive field effect transistor
- urea sensor
- urease
ASJC Scopus subject areas
- Chemistry(all)
- Drug Discovery