Urea Sensor Based on an Ion Sensitive Field Effect Transistor. II.1,2) Effects of Buffer Concentration and pH on the Potentiometric Response

Jun Ichi Anzai, Yoko Ohki, Tetsuo Osa, Hideki Nakajima, Tadayuki Matsuo

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

The effects of pH and concentration of working buffer on the potentiometric response of a urea sensor based on an ion-sensitive field effect transistor (ISFET) were examined. The sensor showed a higher response in slightly acidic buffer solution (pH 6.0) than in buffer solutions of pH 7.1 and 8.0. The pH dependence of the response of the sensor can be explained in terms of the activity of urease immobilized on the ISFET. The buffer concentration also affected the response. Higher concentration buffers (20 and 50 mM) strongly disturbed the response due to the higher buffer capacity.

Original languageEnglish
Pages (from-to)2556-2559
Number of pages4
JournalChemical and Pharmaceutical Bulletin
Volume33
Issue number6
DOIs
Publication statusPublished - 1985 Jan 1

Keywords

  • enzyme sensor
  • immobilized enzyme
  • ion-sensitive field effect transistor
  • urea sensor
  • urease

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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