Upregulation of atrial natriuretic peptide gene expression in remnant kidney of rats with reduced renal mass

Kazuhito Totsune, Harald S. Mackenzie, Hiroko Totsune, Julia L. Troy, Jonathan Lytton, Barry M. Brenner

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

Atrial natriuretic peptide (ANP) is synthesized in the kidney but its physiologic significance there is unclear. To determine whether renal expression of the ANP gene is regulated, renal ANP mRNA expression was assessed in remnant kidneys after 5/6 nephrectomy in Munich-Wistar rats. In normal sodium intake groups, ANP mRNA expression in the remnant kidney was significantly increased by 5.0 ± 0.8-fold (n = 7, mean ± SEM) at 4 d when compared with sham-operated controls (n = 6, all sham-operated groups) ((*)P < 0.001 by Scheffe's test) and by 28.3 ± 5.1-fold at 14 d. This latter response was markedly diminished to 7.6 ± 2.1-fold (n = 7, versus sham) in rats maintained on a low sodium diet. At 4 d, on the other hand, no significant downregulation was observed with dietary sodium restriction. Because natriuretic peptides have previously been shown by us to play a major role in the adaptive responses of remnant nephrons to renal mass ablation, these data suggest that ANP of renal origin may contribute to the overall mechanism for enhancing sodium excretion in the face of declining nephron number.

Original languageEnglish
Pages (from-to)1613-1619
Number of pages7
JournalJournal of the American Society of Nephrology
Volume9
Issue number9
Publication statusPublished - 1998 Sep

ASJC Scopus subject areas

  • Nephrology

Fingerprint Dive into the research topics of 'Upregulation of atrial natriuretic peptide gene expression in remnant kidney of rats with reduced renal mass'. Together they form a unique fingerprint.

Cite this