Abstract
Aberrant messenger RNAs containing a premature termination codon (PTC) are eliminated by the nonsense-mediated mRNA decay (NMD) pathway. Here, we show that a crucial NMD factor, up frameshift 1 protein (Upf1), is required for rapid proteasome-mediated degradation of an aberrant protein (PTC product) derived from a PTC-containing mRNA. Western blot and pulse-chase analyses revealed that Upf1 stimulates the degradation of specific PTC products by the proteasome. Moreover, the Upf1-dependent, proteasome-mediated degradation of the PTC product was also stimulated by mRNAs harbouring a faux 3′ untranslated region (3′-UTR). These results indicate that protein stability might be regulated by an aberrant mRNA 3′-UTR.
Original language | English |
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Pages (from-to) | 1265-1271 |
Number of pages | 7 |
Journal | EMBO Reports |
Volume | 10 |
Issue number | 11 |
DOIs | |
Publication status | Published - 2009 |
Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Genetics