Upf1 stimulates degradation of the product derived from aberrant messenger RNA containing a specific nonsense mutation by the proteasome

Kazushige Kuroha, Tsuyako Tatematsu, Toshifumi Inada

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Aberrant messenger RNAs containing a premature termination codon (PTC) are eliminated by the nonsense-mediated mRNA decay (NMD) pathway. Here, we show that a crucial NMD factor, up frameshift 1 protein (Upf1), is required for rapid proteasome-mediated degradation of an aberrant protein (PTC product) derived from a PTC-containing mRNA. Western blot and pulse-chase analyses revealed that Upf1 stimulates the degradation of specific PTC products by the proteasome. Moreover, the Upf1-dependent, proteasome-mediated degradation of the PTC product was also stimulated by mRNAs harbouring a faux 3′ untranslated region (3′-UTR). These results indicate that protein stability might be regulated by an aberrant mRNA 3′-UTR.

Original languageEnglish
Pages (from-to)1265-1271
Number of pages7
JournalEMBO Reports
Volume10
Issue number11
DOIs
Publication statusPublished - 2009
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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