Up-regulation of ras-GAP genes is reversed by a MEK inhibitor and doxorubicin in v-Ki-ras-transformed NIH/3T3 fibroblasts

Minako Hashii, Mitsunori Fukuda, Hideki Nomura, Naoko Ito, Hiroto Takahashi, Seisuke Hattori, Katsuhiko Mikoshiba, Makoto Noda, Yoshihiro Higuchi

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Ras-GTPase-activating proteins (Ras-GAPs) have been implicated both as suppressors of Ras and as effectors in regulating cellular activities. To study whether Ras-GAPs have roles in tumor cell survival or not, mRNA levels of ras-related genes were measured in v-Ki-ras-transformed (DT) and the parental NIH/3T3 cells, using real-time PCR. mRNA levels of p120-Gap, Gap1m, and PIK3CA were increased in DT cells compared with NIH/3T3 cells. p120-Gap and PIK3CA genes were induced by addition of serum or epidermal growth factor to serum-starved DT cells. Three anti-cancer drugs, an ERK kinase (MEK) inhibitor PD98059, a topoisomerase II poison doxorubicin (adriamycin), and a histone deacetylase inhibitor trichostatin A, selectively blocked the overexpression of p120-Gap and Gap1m genes in DT cells. These drugs also caused reversion of DT cells to the adherent shape associated with growth arrest. Our results suggest that p120-Gap and Gap1m genes provide important biomarkers for cancer therapies.

Original languageEnglish
Pages (from-to)374-380
Number of pages7
JournalBiochemical and biophysical research communications
Volume356
Issue number2
DOIs
Publication statusPublished - 2007 May 4

Keywords

  • Doxorubicin
  • Expression
  • Gap1
  • MEK
  • Trichostatin A
  • ras
  • ras-GAP

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Up-regulation of ras-GAP genes is reversed by a MEK inhibitor and doxorubicin in v-Ki-ras-transformed NIH/3T3 fibroblasts'. Together they form a unique fingerprint.

Cite this