Up-regulation of PSF1 promotes the growth of breast cancer cells

Izumi Nakahara, Mamiko Miyamoto, Tatsuhiro Shibata, Sadako Akashi-Tanaka, Takayuki Kinoshita, Kaoru Mogushi, Kohtaro Oda, Masaya Ueno, Nobuyuki Takakura, Hiroshi Mizushima, Hiroshi Tanaka, Tsutomu Ohta

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

PSF1 is a subunit of the GINS complex that functions along with the MCM2-7 complex and Cdc45 in eukaryotic DNA replication. Although mammalian PSF1 is predominantly expressed in highly proliferating cells and organs, little is known about the roles of PSF1 in mature cells or cancer cells. We found that PSF1 was expressed at relatively high levels in breast tumor cells, but at low levels in normal breast cells. Knockdown of PSF1 expression using small interfering RNA (siRNA) slowed the growth of breast cancer cell lines by delaying DNA replication but did not affect proliferation of normal human mammary epithelial cells. Reduced PSF1 expression also inhibited anchorage-independent growth in breast cancer cell lines. These results suggest that PSF1 over-expression is specifically involved in breast cancer cell growth. Therefore, PSF1 inhibition might provide new therapeutic approaches for breast cancer.

Original languageEnglish
Pages (from-to)1015-1024
Number of pages10
JournalGenes to Cells
Volume15
Issue number10
DOIs
Publication statusPublished - 2010 Oct 1

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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    Nakahara, I., Miyamoto, M., Shibata, T., Akashi-Tanaka, S., Kinoshita, T., Mogushi, K., Oda, K., Ueno, M., Takakura, N., Mizushima, H., Tanaka, H., & Ohta, T. (2010). Up-regulation of PSF1 promotes the growth of breast cancer cells. Genes to Cells, 15(10), 1015-1024. https://doi.org/10.1111/j.1365-2443.2010.01442.x