Up-regulation of protein serine/threonine phosphatase type 2C during 1α,25-dihydroxyvitamin D3-induced monocytic differentiation of leukemic HL-60 cells

Masakatsu Nishikawa; Serdar B. Omay; Keiji Nakai; Hisakazu Kihira; Takayasu Kobayashi; Shinri Tamura; Hiroshi Shiku

doi:10.1016/0014-5793(95)01209-W

Up-regulation of protein serine/threonine phosphatase type 2C during 1α,25-dihydroxyvitamin D₃-induced monocytic differentiation of leukemic HL-60 cells

Masakatsu Nishikawa, Serdar B. Omay, Keiji Nakai, Hisakazu Kihira, Takayasu Kobayashi, Shinri Tamura, Hiroshi Shiku

Center for Gene Research

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Treatment with 20 nM 1α,25-dihydroxyvitamin D₃ (1,25(OH)₂D₃) caused a progressive increase in the activity of Mg²⁺-dependent protein serine/threonine phosphatase type 2C (PP2C) in subcellular fractions of HL-60 cells, whereas PP2C activity was relatively constant throughout all-trans retinoic acid-induced (1 μM) granulocytic differentiation. The increase in PP2C activity appeared to parallel the 1,25(OH)₂D₃-induced phenotypic and functional changes in HL-60 cells. Immunoblot and Northern blot analysis indicated that the increase in PP2C activity corresponded to the increased expression of PP2C protein, which was preceded by an increase in the level of mRNA for PP2Cβ. No mRNA for PP2Cα was detected in resting or 1,25(OH)₂D₃-stimulated HL-60 cells. These results suggest that the increased expression of PP2C is related with the 1,25(OH)₂D₃-induced monocytic differentiation of HL-60 cells.

Original language	English
Pages (from-to)	299-303
Number of pages	5
Journal	FEBS Letters
Volume	375
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(95)01209-W
Publication status	Published - 1995 Nov 20

Keywords

1α,25-Dihydroxyvitamin D
Differentiation
HL-60 cell
Protein phosphatase

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/0014-5793(95)01209-W

Fingerprint Dive into the research topics of 'Up-regulation of protein serine/threonine phosphatase type 2C during 1α,25-dihydroxyvitamin D<sub>3</sub>-induced monocytic differentiation of leukemic HL-60 cells'. Together they form a unique fingerprint.

Cite this

Up-regulation of protein serine/threonine phosphatase type 2C during 1α,25-dihydroxyvitamin D₃-induced monocytic differentiation of leukemic HL-60 cells. / Nishikawa, Masakatsu; Omay, Serdar B.; Nakai, Keiji; Kihira, Hisakazu; Kobayashi, Takayasu; Tamura, Shinri; Shiku, Hiroshi.

In: FEBS Letters, Vol. 375, No. 3, 20.11.1995, p. 299-303.

Research output: Contribution to journal › Article › peer-review

@article{37e10793a4834516a43cac1355a12fc5,

title = "Up-regulation of protein serine/threonine phosphatase type 2C during 1α,25-dihydroxyvitamin D3-induced monocytic differentiation of leukemic HL-60 cells",

abstract = "Treatment with 20 nM 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) caused a progressive increase in the activity of Mg2+-dependent protein serine/threonine phosphatase type 2C (PP2C) in subcellular fractions of HL-60 cells, whereas PP2C activity was relatively constant throughout all-trans retinoic acid-induced (1 μM) granulocytic differentiation. The increase in PP2C activity appeared to parallel the 1,25(OH)2D3-induced phenotypic and functional changes in HL-60 cells. Immunoblot and Northern blot analysis indicated that the increase in PP2C activity corresponded to the increased expression of PP2C protein, which was preceded by an increase in the level of mRNA for PP2Cβ. No mRNA for PP2Cα was detected in resting or 1,25(OH)2D3-stimulated HL-60 cells. These results suggest that the increased expression of PP2C is related with the 1,25(OH)2D3-induced monocytic differentiation of HL-60 cells.",

keywords = "1α,25-Dihydroxyvitamin D, Differentiation, HL-60 cell, Protein phosphatase",

author = "Masakatsu Nishikawa and Omay, {Serdar B.} and Keiji Nakai and Hisakazu Kihira and Takayasu Kobayashi and Shinri Tamura and Hiroshi Shiku",

note = "Funding Information: Acknowledgements: This work was supported by grants for research from the Ministry of Education, Science, and Culture, Japan.",

year = "1995",

month = nov,

day = "20",

doi = "10.1016/0014-5793(95)01209-W",

language = "English",

volume = "375",

pages = "299--303",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Up-regulation of protein serine/threonine phosphatase type 2C during 1α,25-dihydroxyvitamin D3-induced monocytic differentiation of leukemic HL-60 cells

AU - Nishikawa, Masakatsu

AU - Omay, Serdar B.

AU - Nakai, Keiji

AU - Kihira, Hisakazu

AU - Kobayashi, Takayasu

AU - Tamura, Shinri

AU - Shiku, Hiroshi

N1 - Funding Information: Acknowledgements: This work was supported by grants for research from the Ministry of Education, Science, and Culture, Japan.

PY - 1995/11/20

Y1 - 1995/11/20

N2 - Treatment with 20 nM 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) caused a progressive increase in the activity of Mg2+-dependent protein serine/threonine phosphatase type 2C (PP2C) in subcellular fractions of HL-60 cells, whereas PP2C activity was relatively constant throughout all-trans retinoic acid-induced (1 μM) granulocytic differentiation. The increase in PP2C activity appeared to parallel the 1,25(OH)2D3-induced phenotypic and functional changes in HL-60 cells. Immunoblot and Northern blot analysis indicated that the increase in PP2C activity corresponded to the increased expression of PP2C protein, which was preceded by an increase in the level of mRNA for PP2Cβ. No mRNA for PP2Cα was detected in resting or 1,25(OH)2D3-stimulated HL-60 cells. These results suggest that the increased expression of PP2C is related with the 1,25(OH)2D3-induced monocytic differentiation of HL-60 cells.

AB - Treatment with 20 nM 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) caused a progressive increase in the activity of Mg2+-dependent protein serine/threonine phosphatase type 2C (PP2C) in subcellular fractions of HL-60 cells, whereas PP2C activity was relatively constant throughout all-trans retinoic acid-induced (1 μM) granulocytic differentiation. The increase in PP2C activity appeared to parallel the 1,25(OH)2D3-induced phenotypic and functional changes in HL-60 cells. Immunoblot and Northern blot analysis indicated that the increase in PP2C activity corresponded to the increased expression of PP2C protein, which was preceded by an increase in the level of mRNA for PP2Cβ. No mRNA for PP2Cα was detected in resting or 1,25(OH)2D3-stimulated HL-60 cells. These results suggest that the increased expression of PP2C is related with the 1,25(OH)2D3-induced monocytic differentiation of HL-60 cells.

KW - 1α,25-Dihydroxyvitamin D

KW - Differentiation

KW - HL-60 cell

KW - Protein phosphatase

UR - http://www.scopus.com/inward/record.url?scp=0028851943&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028851943&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(95)01209-W

DO - 10.1016/0014-5793(95)01209-W

M3 - Article

C2 - 7498522

AN - SCOPUS:0028851943

VL - 375

SP - 299

EP - 303

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 3

ER -