Abstract
The biosynthetic machinery of the first fungal ribosomally synthesized and post-translationally modified peptide (RiPP) ustiloxin B was elucidated through a series of gene inactivation and heterologous expression studies. The results confirmed an essential requirement for novel oxidases possessing the DUF3328 motif for macrocyclization, and highly unique side-chain modifications by three oxidases (UstCF1F2) and a pyridoxal 5′-phosphate (PLP)-dependent enzyme (UstD). These findings provide new insight into the expression of the RiPP gene clusters found in various fungi.
Original language | English |
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Pages (from-to) | 8072-8075 |
Number of pages | 4 |
Journal | Angewandte Chemie - International Edition |
Volume | 55 |
Issue number | 28 |
DOIs | |
Publication status | Published - 2016 Jul 4 |
Externally published | Yes |
Keywords
- RiPPs
- biosynthesis
- heterologous expression
- natural products
- ustiloxins
ASJC Scopus subject areas
- Catalysis
- Chemistry(all)