Unveiling the Biosynthetic Pathway of the Ribosomally Synthesized and Post-translationally Modified Peptide Ustiloxin B in Filamentous Fungi

Ying Ye, Atsushi Minami, Yuya Igarashi, Miho Izumikawa, Myco Umemura, Nozomi Nagano, Masayuki Machida, Teppei Kawahara, Kazuo Shin-ya, Katsuya Gomi, Hideaki Oikawa

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The biosynthetic machinery of the first fungal ribosomally synthesized and post-translationally modified peptide (RiPP) ustiloxin B was elucidated through a series of gene inactivation and heterologous expression studies. The results confirmed an essential requirement for novel oxidases possessing the DUF3328 motif for macrocyclization, and highly unique side-chain modifications by three oxidases (UstCF1F2) and a pyridoxal 5′-phosphate (PLP)-dependent enzyme (UstD). These findings provide new insight into the expression of the RiPP gene clusters found in various fungi.

Original languageEnglish
Pages (from-to)8072-8075
Number of pages4
JournalAngewandte Chemie - International Edition
Volume55
Issue number28
DOIs
Publication statusPublished - 2016 Jan 1

Keywords

  • RiPPs
  • biosynthesis
  • heterologous expression
  • natural products
  • ustiloxins

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Fingerprint Dive into the research topics of 'Unveiling the Biosynthetic Pathway of the Ribosomally Synthesized and Post-translationally Modified Peptide Ustiloxin B in Filamentous Fungi'. Together they form a unique fingerprint.

  • Cite this

    Ye, Y., Minami, A., Igarashi, Y., Izumikawa, M., Umemura, M., Nagano, N., Machida, M., Kawahara, T., Shin-ya, K., Gomi, K., & Oikawa, H. (2016). Unveiling the Biosynthetic Pathway of the Ribosomally Synthesized and Post-translationally Modified Peptide Ustiloxin B in Filamentous Fungi. Angewandte Chemie - International Edition, 55(28), 8072-8075. https://doi.org/10.1002/anie.201602611