Unveiling the Biosynthetic Pathway of the Ribosomally Synthesized and Post-translationally Modified Peptide Ustiloxin B in Filamentous Fungi

Ying Ye; Atsushi Minami; Yuya Igarashi; Miho Izumikawa; Myco Umemura; Nozomi Nagano; Masayuki Machida; Teppei Kawahara; Kazuo Shin-ya; Katsuya Gomi; Hideaki Oikawa

doi:10.1002/anie.201602611

Unveiling the Biosynthetic Pathway of the Ribosomally Synthesized and Post-translationally Modified Peptide Ustiloxin B in Filamentous Fungi

Ying Ye, Atsushi Minami, Yuya Igarashi, Miho Izumikawa, Myco Umemura, Nozomi Nagano, Masayuki Machida, Teppei Kawahara, Kazuo Shin-ya, Katsuya Gomi, Hideaki Oikawa

AGR - Bioscience and Biotechnology for Future Bioindustries

Research output: Contribution to journal › Article

32 Citations (Scopus)

Abstract

The biosynthetic machinery of the first fungal ribosomally synthesized and post-translationally modified peptide (RiPP) ustiloxin B was elucidated through a series of gene inactivation and heterologous expression studies. The results confirmed an essential requirement for novel oxidases possessing the DUF3328 motif for macrocyclization, and highly unique side-chain modifications by three oxidases (UstCF1F2) and a pyridoxal 5′-phosphate (PLP)-dependent enzyme (UstD). These findings provide new insight into the expression of the RiPP gene clusters found in various fungi.

Original language	English
Pages (from-to)	8072-8075
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	55
Issue number	28
DOIs	https://doi.org/10.1002/anie.201602611
Publication status	Published - 2016 Jan 1

Keywords

RiPPs
biosynthesis
heterologous expression
natural products
ustiloxins

ASJC Scopus subject areas

Catalysis
Chemistry(all)

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Ye, Y., Minami, A., Igarashi, Y., Izumikawa, M., Umemura, M., Nagano, N., Machida, M., Kawahara, T., Shin-ya, K., Gomi, K., & Oikawa, H. (2016). Unveiling the Biosynthetic Pathway of the Ribosomally Synthesized and Post-translationally Modified Peptide Ustiloxin B in Filamentous Fungi. Angewandte Chemie - International Edition, 55(28), 8072-8075. https://doi.org/10.1002/anie.201602611

Unveiling the Biosynthetic Pathway of the Ribosomally Synthesized and Post-translationally Modified Peptide Ustiloxin B in Filamentous Fungi. / Ye, Ying; Minami, Atsushi; Igarashi, Yuya; Izumikawa, Miho; Umemura, Myco; Nagano, Nozomi; Machida, Masayuki; Kawahara, Teppei; Shin-ya, Kazuo; Gomi, Katsuya; Oikawa, Hideaki.

In: Angewandte Chemie - International Edition, Vol. 55, No. 28, 01.01.2016, p. 8072-8075.

Research output: Contribution to journal › Article

Ye, Y, Minami, A, Igarashi, Y, Izumikawa, M, Umemura, M, Nagano, N, Machida, M, Kawahara, T, Shin-ya, K, Gomi, K & Oikawa, H 2016, 'Unveiling the Biosynthetic Pathway of the Ribosomally Synthesized and Post-translationally Modified Peptide Ustiloxin B in Filamentous Fungi', Angewandte Chemie - International Edition, vol. 55, no. 28, pp. 8072-8075. https://doi.org/10.1002/anie.201602611

Ye, Ying ; Minami, Atsushi ; Igarashi, Yuya ; Izumikawa, Miho ; Umemura, Myco ; Nagano, Nozomi ; Machida, Masayuki ; Kawahara, Teppei ; Shin-ya, Kazuo ; Gomi, Katsuya ; Oikawa, Hideaki. / Unveiling the Biosynthetic Pathway of the Ribosomally Synthesized and Post-translationally Modified Peptide Ustiloxin B in Filamentous Fungi. In: Angewandte Chemie - International Edition. 2016 ; Vol. 55, No. 28. pp. 8072-8075.

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