Unsaturated fatty acids promote proliferation via ERK1/2 and Akt pathway in bovine mammary epithelial cells

Tomo Yonezawa, Satoshi Haga, Yosuke Kobayashi, Kazuo Katoh, Yoshiaki Obara

    Research output: Contribution to journalArticle

    53 Citations (Scopus)

    Abstract

    GPR40 has recently been identified as a G protein-coupled cell-surface receptor for long-chain fatty acids (LCFAs). The mRNA of the bovine ortholog of GPR40 (bGPR40) was detected by RT-PCR in cloned bovine mammary epithelial cells (bMEC) and in the bovine mammary gland at various stages of lactation. Oleate and linoleate caused an increase in intracellular Ca2+ concentrations in these cells, and significantly reduced forskolin-induced cAMP concentrations. Phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 and Akt kinase, which regulates cell proliferation and survival, was rapidly increased by oleate. Incubation with oleate and linoleate for 24 h significantly promoted cell proliferation. Moreover, in serum-free medium, oleate significantly stimulated cell proliferation during a 7-day culture. These results suggest that bGPR40 mediates LCFA signaling in mammary epithelial cells and thereby plays an important role in cell proliferation and survival.

    Original languageEnglish
    Pages (from-to)729-735
    Number of pages7
    JournalBiochemical and biophysical research communications
    Volume367
    Issue number4
    DOIs
    Publication statusPublished - 2008 Mar 21

    Keywords

    • Long-chain fatty acid receptors
    • Long-chain fatty acids
    • Mammary epithelial cells

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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