Unlabeled imaging of primary cilia by scanning ion conductance microscopy

Yuanshu Zhou, Masaki Saito, Takeshi Fukuma, Yasufumi Takahashi

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Primary cilia are non-motile cilia consisting of a centriole-derived basal body and a microtubule-based axoneme. In recent years, the structure and function of primary cilia have been attracting attention due to the relation with the onset of ciliary disease. Scanning ion conductance microscopy (SICM) is a probe microscopy used to measure the topography and functions of living cells at nanoscale. Furthermore, the labelling procedure is not necessary for SICM measurement compare to fluorescence imaging. We compared the structures of primary cilia of human retinal pigment epithelial cell line (RPE-1 cells) and Madin-Darby canine kidney cell line (MDCK cells) at nanoscale by using SICM. In addition, high resolution SICM images have also succeeded in visualizing ciliary pockets that difficult to be fluorescently labeled.

    Original languageEnglish
    Pages (from-to)192-196
    Number of pages5
    JournalFolia Pharmacologica Japonica
    Volume154
    Issue number4
    DOIs
    Publication statusPublished - 2019

    ASJC Scopus subject areas

    • Pharmacology

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