Unique characteristics of superoxide dismutase of a strictly anaerobic archaebacterium methanobacterium thermoautotrophicum

Masashi Takao, Akira Yasui, Atsushi Oikawa

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53 Citations (Scopus)

Abstract

The Superoxide dismutase (SOD) gene of Methanobacterium thermoautotrophicum (Takao, M., Oikawa, A., and Yasui, A. (1990) Arch. Biochem. Biophys. 283, 210-216), a strictly anaerobic archaebacterium, was expressed in Escherichia coli. The gene product accounted for more than 30% of the host's soluble protein. The purified protein was an active iron-containing tetrameric SOD with specific activity similar to known manganese-containing SODs (MnSODs) of aerobic archaebacteria. Although M. thermoautotrophicum SOD is an iron-containing SOD (FeSOD), it resembles MnSODs in amino acid sequence as judged by criteria distinguishing FeSODs from MnSODs. Moreover, M. thermoautotrophicum SOD is resistant to azide and hydrogen peroxide as MnSODs are, suggesting that its evolution is distinct from known eubacterial FeSODs.

Original languageEnglish
Pages (from-to)14151-14154
Number of pages4
JournalJournal of Biological Chemistry
Volume266
Issue number22
Publication statusPublished - 1991 Dec 1

ASJC Scopus subject areas

  • Biochemistry

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