Uniform accumulation of recombinant miraculin protein in transgenic tomato fruit using a fruit-ripening-specific E8 promoter

Tadayoshi Hirai, You Wang Kim, Kazuhisa Kato, Kyoko Hiwasa-Tanase, Hiroshi Ezura

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

The E8 promoter, a tomato fruit-ripening-specific promoter, and the CaMV 35S promoter, a constitutive promoter, were used to express the miraculin gene encoding the taste-modifying protein in tomato. The accumulation of miraculin protein and mRNA was compared among transgenic tomatoes expressing the miraculin gene driven by these promoters. Recombinant miraculin protein predominantly accumulated in transgenic tomato lines using the E8 promoter (E8-MIR) only at the red fruit stage. The accumulations were almost uniform among all fruit tissues. When the 35S promoter (35S-MIR) was used, miraculin accumulation in the exocarp was much higher than in other tissues, indicating that the miraculin accumulation pattern can be regulated by using different types of promoters. We also discuss the potential of the E8-MIR lines for practical use.

Original languageEnglish
Pages (from-to)1285-1292
Number of pages8
JournalTransgenic Research
Volume20
Issue number6
DOIs
Publication statusPublished - 2011 Dec
Externally publishedYes

Keywords

  • E8 promoter
  • Miraculin
  • Taste-modifying protein
  • Tomato

ASJC Scopus subject areas

  • Biotechnology
  • Animal Science and Zoology
  • Agronomy and Crop Science
  • Genetics

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