Unidentified compound specifically accumulated in soybean nodules formed with h2-uptake negative rhizobium japonicum strains

Yasuhiro Arima, Kikuo Kumazawa, Kiwamu Minamisawa

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

A large quantity of an unidentified compound was detected by a fluorogenic method in the soybean nodules formed with H2-uptake negative Rhizobium japonicum strains. The unidentified compound (compound X) reacted with o-phthalaldehyde in the presence of 2-mercaptoethanol giving rise to a fluorescent compound, and was not hydrolyzed in strong acid and strong base. Therefore, the compound X was considered to be one of amines and amino acids. The content of the compound X in the nodules formed with H2-uptake positive strains was remarkably lower than that in the nodules formed with H2-uptake negative strains. It was suggested that the compound X may be involved in the process of hydrogen metabolism in soybean nodules. The compound X was not detected in leaves, stems, petioles, and roots of soybean plants, but detected in H2-uptake negative strains of free-living R. japonicum. In nodules, a great portion of the compound X was distributed into cytosol fraction.

Original languageEnglish
Pages (from-to)435-444
Number of pages10
JournalSoil Science and Plant Nutrition
Volume30
Issue number3
DOIs
Publication statusPublished - 1984 Sep
Externally publishedYes

Keywords

  • Amines
  • Amino acid analysis
  • Fluorogenic detection
  • Hydrogenase

ASJC Scopus subject areas

  • Soil Science
  • Plant Science

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