Ultraviolet light-mediated photofunctionalization of titanium to promote human mesenchymal stem cell migration, attachment, proliferation and differentiation

Hideki Aita, Wael Att, Takeshi Ueno, Masahiro Yamada, Norio Hori, Fuminori Iwasa, Naoki Tsukimura, Takahiro Ogawa

Research output: Contribution to journalArticlepeer-review

139 Citations (Scopus)


Improving the osteoconductive potential of titanium implants has been of continuing interest in the fields of dentistry and orthopedic surgery. This study determined the bioactivity of ultraviolet (UV) light-treated titanium. Human mesenchymal stem cells (MSCs) were cultured on acid-etched microtopographical titanium surfaces with and without 48 h pretreatment with UVA (peak wavelength of 360 nm) or UVC (peak wavelength of 250 nm). The number of cells that migrated to the UVC-treated surface during the first 3 h of incubation was eight times higher than those that migrated to the untreated surface. After 24 h of incubation, the number of cells attached to the UVC-treated surface was over three times more than those attached to the untreated surface. On the UVC-treated surface, the cellular spread was expedited with an extensive and intensive expression of the focal adhesion protein vinculin. The cells on the UVC-treated surface exhibited a threefold higher bromodeoxyuridine incorporation, a doubling of the alkaline phosphatase-positive area and the up-regulated expression of bone-related genes, indicating the accelerated proliferation and differentiation. The UVC-treated surface did not adversely affect the viability of the cells. These biological effects were not seen after UVA treatment, despite the generation of superhydrophilicity. Thus, we discovered a novel photofunctionalization of titanium dioxide that substantially enhances its bioactivity in human MSCs. Further studies are required to investigate the universal effectiveness of this surface modification for different titanium-containing materials, with varying chemistries and textures, as well as to understand its significance in enhancing in vivo osteoconductivity.

Original languageEnglish
Pages (from-to)3247-3257
Number of pages11
JournalActa Biomaterialia
Issue number8
Publication statusPublished - 2009 Oct
Externally publishedYes


  • Orthopedic and dental implant
  • Osseointegration
  • Osteoblast
  • Superhydrophilicity
  • UVC

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology


Dive into the research topics of 'Ultraviolet light-mediated photofunctionalization of titanium to promote human mesenchymal stem cell migration, attachment, proliferation and differentiation'. Together they form a unique fingerprint.

Cite this