TY - JOUR
T1 - Ultraviolet-B sensitivities in Japanese lowland rice cultivars
T2 - Cyclobutane pyrimidine dimer photolyase activity and gene mutation
AU - Teranishi, Mika
AU - Iwamatsu, Yutaka
AU - Hidema, Jun
AU - Kumagai, Tadashi
N1 - Funding Information:
The authors are grateful Dr. Kuniaki Nagano (Miyagi Prefecture Furukawa Agricultural Experiment Station, Miyagi, Japan) and Dr. Tadashi Sato (Graduate School of Life Sciences, Tohoku University) for providing seeds of rice cultivars, and Drs. Betsy M. Sutherland and Benjamin Burr (Brookhaven National Laboratory, New York, U.S.A.) for critical reading of this manuscript. This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (16710028 to M.T., 14704060 to J.H. and 15201010 and 16651019 to T.K., respectively).
PY - 2004/12
Y1 - 2004/12
N2 - There is a cultivar difference in the response to ultraviolet-B (UVB: 280-320 nm) in rice (Oryza sativa L.). Among Japanese lowland rice cultivars, Sasanishiki, a leading Japanese rice cultivar, is resistant to the damaging effects of UVB while Norin 1, a close relative, is less resistant. We found previously that Norin 1 was deficient in cyclobutane pyrimidine dimer (CPD) photorepair ability and suggested that the UVB sensitivity in rice depends largely on CPD photorepair ability. In order to verify that suggestion, we examined the correlation between UVB sensitivity and CPD photolyase activity in 17 rice cultivars of progenitors and relatives in breeding of UV-resistant Sasanishiki and UV-sensitive Norin 1. The amino acid at position 126 of the deduced amino acid sequence of CPD photolyase in cultivars including such as Norin 1 was found to be arginine, the CPD photolyase activities of which were lower. The amino acid at that position in cultivars including such as Sasanishiki was glutamine. Furthermore, cultivars more resistant to UVB were found to exhibit higher photolyase activities than less resistant cultivars. These results emphasize that single amino acid alteration from glutamine to arginine leads to a deficit of CPD photolyase activity and that CPD photolyase activity is one of the main factors determining UVB sensitivity in rice.
AB - There is a cultivar difference in the response to ultraviolet-B (UVB: 280-320 nm) in rice (Oryza sativa L.). Among Japanese lowland rice cultivars, Sasanishiki, a leading Japanese rice cultivar, is resistant to the damaging effects of UVB while Norin 1, a close relative, is less resistant. We found previously that Norin 1 was deficient in cyclobutane pyrimidine dimer (CPD) photorepair ability and suggested that the UVB sensitivity in rice depends largely on CPD photorepair ability. In order to verify that suggestion, we examined the correlation between UVB sensitivity and CPD photolyase activity in 17 rice cultivars of progenitors and relatives in breeding of UV-resistant Sasanishiki and UV-sensitive Norin 1. The amino acid at position 126 of the deduced amino acid sequence of CPD photolyase in cultivars including such as Norin 1 was found to be arginine, the CPD photolyase activities of which were lower. The amino acid at that position in cultivars including such as Sasanishiki was glutamine. Furthermore, cultivars more resistant to UVB were found to exhibit higher photolyase activities than less resistant cultivars. These results emphasize that single amino acid alteration from glutamine to arginine leads to a deficit of CPD photolyase activity and that CPD photolyase activity is one of the main factors determining UVB sensitivity in rice.
KW - CPD photolyase
KW - Cyclobutane pyrimidine dimer (CPD)
KW - Gene mutation
KW - Rice (oryza sative L.)
KW - UVB radiation
KW - UVB sensitivity
UR - http://www.scopus.com/inward/record.url?scp=12444276781&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=12444276781&partnerID=8YFLogxK
U2 - 10.1093/pcp/pch215
DO - 10.1093/pcp/pch215
M3 - Article
C2 - 15653803
AN - SCOPUS:12444276781
VL - 45
SP - 1848
EP - 1856
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 12
ER -