Ultrastructure of sarcoma 180 cells after ultrasound irradiation in the presence of sparfloxacin

Deqing Huang, Kyoji Okada, Chiyo Komori, Eiji Itoi, Koichi Kawamura, Toshio Suzuki

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Background: Sonodynamic synergistic antitumor effects have been well described to date, but detailed ultrastructural studies in this field are still lacking. Materials and Methods: A suspension of sarcoma 180 cells was exposed to ultrasound (US) in the presence of sparfloxacin (SPFX), one of the new quinolone antibiotics, at 2 W (0.64 W/cm2) for 30 seconds. The antitumor effect was evaluated by the survival rate of cells and cell morphology by scanning and transmission electron microscopy. Results: After US irradiation, the survival rate of tumor cells in the SPFX-added group was 30.9%, significantly lower than 78.7% in the control group (p=0.0002). Most cells in the control group were spherical, but in the SPFX-added group the cells were aspherical. Cell membranes were often ruptured and numerous pores of various sizes were observed. Some cells were totally disintegrated. Conclusion: The most characteristic changes of the synergistic antitumor activities of US and SPFX were on the cell membrane.

Original languageEnglish
Pages (from-to)1553-1559
Number of pages7
JournalAnticancer research
Volume24
Issue number3 A
Publication statusPublished - 2004 May
Externally publishedYes

Keywords

  • Sarcoma 180 cell ultrastructure
  • Sparfloxacin
  • Ultrasound irradiation

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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