Ultrastructure of papillar cells in brassica campestris revealed by liquid helium rapid-freezing and substitution-fixation method

Naoko Kishi-nishizawa, Akira Isogai, Masao Watanabe, Kokichi Hinata, Seiei Yamakawa, Shinsuke Shojima, Akinori Suzuki

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

The ultrastructure of papillar cells of Brassica campestris on the day of anthesis was studied by the liquid helium rapid-freezing and a substitution-fixation method, abbreviated as the RFS method. Application of the RFS method to the analysis of papillar cells enabled us to examine clear images of these cells which have not been observed previously. The well-developed rough and smooth endoplasmic reticulum, numerous Golgi bodies and mitochondria, various small vesicles and clathrin-coated vesicles, were present in the cells. The numbers of Golgi bodies, as well as the numbers of cisternae of each Golgi body, increased as compared to that in the other cells of style. Lattice-like fenestrated and flattened cisternae were seen adjacent to the narrowest trans cisterna of the Golgi body, which had a partially coated region at its periphery. Many coated vesicles were observed in the vicinity of this structure and the plasma membrane. Coated areas on the plasma membrane were also observed. The ultrastructure of papillar cells on the day of anthesis indicated that they are very active secretory cells. By using an antibody against S8-protein and sections prepared by the RFS method, we demonstrated the distribution of S8-protein in the cell wall of papillar cells of homozygous plants of Brassica campestris SgS8.

Original languageEnglish
Pages (from-to)1207-1219
Number of pages13
JournalPlant and Cell Physiology
Volume31
Issue number8
Publication statusPublished - 1990

Keywords

  • Brassica campestris
  • Liquid helium rapid-freezing
  • Papillar cell
  • S-protein
  • Secretion
  • Self incompatibility

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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