Ultrasonically induced cell damage enhanced by photofrin II: Mechanism of sonodynamic activation

N. Yumita, S. Umemura, R. Nishigaki

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)


Ultrasonically induced cell damage and active oxygen generation with photofrin II (PF) were compared in the same in vitro insonation setup. Materials and methods: Sarcoma 180 cells suspended in air-saturated PBS were exposed to ultrasound for up to 60 seconds in the presence and absence of PF. The viability was determined by the Trypan Blue exclusion test. Ultrasonically induced active oxygen generation in the presence and absence of PF in air-saturated aqueous solutions of 50 mM 2,2,6,6-tetramethyl-4-piperidone was detected by electron spin resonance (ESR) spectrum. Results: Significant enhancement of the rates of both ultrasonically induced cell damage and nitroxide generation was demonstrated with 20-80 μg/ml PF. Both rates correlated very well. The enhancement of both rates with PF was suppressed by 10 mM histidine. Conclusion: These results may suggest that ultrasonically generated active oxygen plays a primary role in ultrasonically induced cell damage in the presence of PF.

Original languageEnglish
Pages (from-to)425-429
Number of pages5
JournalIn Vivo
Issue number3
Publication statusPublished - 2000


  • Ultrasound, sonodynamic therapy, cell damage, sonodynamic activation, active oxygen, photofrin II

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology


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