Ultrasonically induced cell damage and membrane lipid peroxidation by photofrin II: Mechanism of sonodynamic activation

Nagahiko Yumita, Shinichiro Umemura

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Purpose. Ultrasonically induced cell damage and active oxygen generation with photofrin II were compared using the same in vitro insonation setup. Methods. Sarcoma 180 cells suspended in an air-saturated phosphate-buffered saline solution were exposed to ultrasound at 2MHz for not more than 60s in the presence and absence of photofrin II. The trypan blue exclusion test was used to determine viability. Lipid peroxidation in cell membranes was estimated by measuring the quantity of reactive substance produced from thiobarbituric acid added immediately after the cells had been exposured to ultrasound. Results. Significant enhancement of the rates of ultrasonically induced cell damage and lipid peroxidation was demonstrated with photofrin II (20-80 μg/ml), and the two rates were closely associated. Enhancement of both rates by photofrin II was suppressed by 10mM of histidine. Conclusion. These results suggest that ultrasonically generated active oxygen is a primary factor in ultrasonically induced cell damage in the presence of photofrin II.

Original languageEnglish
Pages (from-to)35-40
Number of pages6
JournalJournal of Medical Ultrasonics
Volume31
Issue number1
DOIs
Publication statusPublished - 2004 Mar 1

Keywords

  • Active oxygen
  • Cell damage
  • Lipid peroxidation
  • Photofrin II
  • Ultrasound

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'Ultrasonically induced cell damage and membrane lipid peroxidation by photofrin II: Mechanism of sonodynamic activation'. Together they form a unique fingerprint.

Cite this