Ultrasonically induced cell damage and active oxygen generation by 4-formyloximeetylidene-3-hydroxyl-2-vinyl-deuterio-porphynyl(IX)-6-7- diaspartic acid: On the mechanism of sonodynamic activation

Nagahiko Yumita, Isao Sakata, Susumu Nakajima, Shinichiro Umemura

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

Ultrasonically induced cell damage and active oxygen generation with 4-formyloximeetylidene-3-hydroxyl-2-vinyl-deuterio-porphynyl(IX)-6-7- diaspartic acid (ATX-S10) were compared in the same in vitro insonation setup. Sarcoma 180 cells suspended in air-saturated PBS were exposed to ultrasound at 2 MHz for up to 60 s in the presence and absence of ATX-S10. The viability was determined by Trypan blue exclusion test. Ultrasonically induced active oxygen generation in the presence and absence of ATX-S10 in air-saturated aqueous solutions of 50 mM 2,2,6,6-tetramethyl-4-piperidone was detected by electron spin resonance (ESR). Significant enhancement of the rates of both ultrasonically induced cell damage and nitroxide generation was demonstrated with 40-160 μM ATX-S10. Both rates correlated very well. The enhancement of both rates with ATX-S10 was suppressed by 10 mM histidine. These results suggest that ultrasonically generated active oxygen plays a primary role in the ultrasonically induced cell damage in the presence of ATX-S10.

Original languageEnglish
Pages (from-to)179-184
Number of pages6
JournalBiochimica et Biophysica Acta - General Subjects
Volume1620
Issue number1-3
DOIs
Publication statusPublished - 2003 Mar 17

Keywords

  • ATX-S10
  • Active oxygen
  • Cell damage
  • Electron spin resonance
  • Sonodynamic activation
  • Ultrasound

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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