The development of technologies for the in vitro amplification of abnormal conformations of prion protein (PrP Sc) has generated the potential for sensitive detection of prions. Here we developed a new PrP Sc amplification assay, called real-time quaking-induced conversion (RT-QUIC), which allows the detection of ≥1 fg of PrP Sc in diluted Creutzfeldt-Jakob disease (CJD) brain homogenate. Moreover, we assessed the technique first in a series of Japanese subjects and then in a blind study of 30 cerebrospinal fluid specimens from Australia, which achieved greater than 80% sensitivity and 100% specificity. These findings indicate the promising enhanced diagnostic capacity of RT-QUIC in the antemortem evaluation of suspected CJD.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)