Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion

Ryuichiro Atarashi, Katsuya Satoh, Kazunori Sano, Takayuki Fuse, Naohiro Yamaguchi, Daisuke Ishibashi, Takehiro Matsubara, Takehiro Nakagaki, Hitoki Yamanaka, Susumu Shirabe, Masahito Yamada, Hidehiro Mizusawa, Tetsuyuki Kitamoto, Genevieve Klug, Amelia McGlade, Steven J. Collins, Noriyuki Nishida

Research output: Contribution to journalArticle

302 Citations (Scopus)

Abstract

The development of technologies for the in vitro amplification of abnormal conformations of prion protein (PrP Sc) has generated the potential for sensitive detection of prions. Here we developed a new PrP Sc amplification assay, called real-time quaking-induced conversion (RT-QUIC), which allows the detection of ≥1 fg of PrP Sc in diluted Creutzfeldt-Jakob disease (CJD) brain homogenate. Moreover, we assessed the technique first in a series of Japanese subjects and then in a blind study of 30 cerebrospinal fluid specimens from Australia, which achieved greater than 80% sensitivity and 100% specificity. These findings indicate the promising enhanced diagnostic capacity of RT-QUIC in the antemortem evaluation of suspected CJD.

Original languageEnglish
Pages (from-to)175-178
Number of pages4
JournalNature Medicine
Volume17
Issue number2
DOIs
Publication statusPublished - 2011 Feb

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Atarashi, R., Satoh, K., Sano, K., Fuse, T., Yamaguchi, N., Ishibashi, D., Matsubara, T., Nakagaki, T., Yamanaka, H., Shirabe, S., Yamada, M., Mizusawa, H., Kitamoto, T., Klug, G., McGlade, A., Collins, S. J., & Nishida, N. (2011). Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion. Nature Medicine, 17(2), 175-178. https://doi.org/10.1038/nm.2294