Ultrafast photoinduced reaction dynamics of photoactive yellow protein (PYP): Observation of coherent oscillations in the femtosecond fluorescence decay dynamics

Noboru Mataga, Haik Chosrowjan, Yutaka Shibata, Yasushi Imamoto, Mikio Kataoka, Fumio Tokunaga

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

We have observed for the first time coherent oscillations in the fs fluorescence (Fl) dynamics of wild-type (w-t) photoactive yellow protein (PYP) and its site-directed mutants. Two oscillatory modes coupled with the ultrafast Fl decay due to the twisting of the excited chromophore were identified, a high frequency mode (∼ 140 cm-1) with 700 fs damping time and a low frequency mode (∼ 50 cm-1) with 500 fs damping time, respectively. The latter becomes overdamped when the structure of the protein nanospace (PNS) surrounding the chromophore becomes more disordered by mutation. Both modes disappear in the F1 dynamics of denatured PYP. Similarities between PYP and rhodopsins (Rh) are discussed.

Original languageEnglish
Pages (from-to)220-225
Number of pages6
JournalChemical Physics Letters
Volume352
Issue number3-4
DOIs
Publication statusPublished - 2002 Jan 30
Externally publishedYes

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

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