Ultrafast photoinduced reaction dynamics of photoactive yellow protein (PYP): Observation of coherent oscillations in the femtosecond fluorescence decay dynamics

Noboru Mataga; Haik Chosrowjan; Yutaka Shibata; Yasushi Imamoto; Mikio Kataoka; Fumio Tokunaga

doi:10.1016/S0009-2614(01)01448-8

Ultrafast photoinduced reaction dynamics of photoactive yellow protein (PYP): Observation of coherent oscillations in the femtosecond fluorescence decay dynamics

Noboru Mataga, Haik Chosrowjan, Yutaka Shibata, Yasushi Imamoto, Mikio Kataoka, Fumio Tokunaga

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

We have observed for the first time coherent oscillations in the fs fluorescence (Fl) dynamics of wild-type (w-t) photoactive yellow protein (PYP) and its site-directed mutants. Two oscillatory modes coupled with the ultrafast Fl decay due to the twisting of the excited chromophore were identified, a high frequency mode (∼ 140 cm^-1) with 700 fs damping time and a low frequency mode (∼ 50 cm^-1) with 500 fs damping time, respectively. The latter becomes overdamped when the structure of the protein nanospace (PNS) surrounding the chromophore becomes more disordered by mutation. Both modes disappear in the F1 dynamics of denatured PYP. Similarities between PYP and rhodopsins (Rh) are discussed.

Original language	English
Pages (from-to)	220-225
Number of pages	6
Journal	Chemical Physics Letters
Volume	352
Issue number	3-4
DOIs	https://doi.org/10.1016/S0009-2614(01)01448-8
Publication status	Published - 2002 Jan 30
Externally published	Yes

ASJC Scopus subject areas

Physics and Astronomy(all)
Physical and Theoretical Chemistry

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10.1016/S0009-2614(01)01448-8

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title = "Ultrafast photoinduced reaction dynamics of photoactive yellow protein (PYP): Observation of coherent oscillations in the femtosecond fluorescence decay dynamics",

abstract = "We have observed for the first time coherent oscillations in the fs fluorescence (Fl) dynamics of wild-type (w-t) photoactive yellow protein (PYP) and its site-directed mutants. Two oscillatory modes coupled with the ultrafast Fl decay due to the twisting of the excited chromophore were identified, a high frequency mode (∼ 140 cm-1) with 700 fs damping time and a low frequency mode (∼ 50 cm-1) with 500 fs damping time, respectively. The latter becomes overdamped when the structure of the protein nanospace (PNS) surrounding the chromophore becomes more disordered by mutation. Both modes disappear in the F1 dynamics of denatured PYP. Similarities between PYP and rhodopsins (Rh) are discussed.",

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T1 - Ultrafast photoinduced reaction dynamics of photoactive yellow protein (PYP)

T2 - Observation of coherent oscillations in the femtosecond fluorescence decay dynamics

AU - Mataga, Noboru

AU - Chosrowjan, Haik

AU - Shibata, Yutaka

AU - Imamoto, Yasushi

AU - Kataoka, Mikio

AU - Tokunaga, Fumio

PY - 2002/1/30

Y1 - 2002/1/30

N2 - We have observed for the first time coherent oscillations in the fs fluorescence (Fl) dynamics of wild-type (w-t) photoactive yellow protein (PYP) and its site-directed mutants. Two oscillatory modes coupled with the ultrafast Fl decay due to the twisting of the excited chromophore were identified, a high frequency mode (∼ 140 cm-1) with 700 fs damping time and a low frequency mode (∼ 50 cm-1) with 500 fs damping time, respectively. The latter becomes overdamped when the structure of the protein nanospace (PNS) surrounding the chromophore becomes more disordered by mutation. Both modes disappear in the F1 dynamics of denatured PYP. Similarities between PYP and rhodopsins (Rh) are discussed.

AB - We have observed for the first time coherent oscillations in the fs fluorescence (Fl) dynamics of wild-type (w-t) photoactive yellow protein (PYP) and its site-directed mutants. Two oscillatory modes coupled with the ultrafast Fl decay due to the twisting of the excited chromophore were identified, a high frequency mode (∼ 140 cm-1) with 700 fs damping time and a low frequency mode (∼ 50 cm-1) with 500 fs damping time, respectively. The latter becomes overdamped when the structure of the protein nanospace (PNS) surrounding the chromophore becomes more disordered by mutation. Both modes disappear in the F1 dynamics of denatured PYP. Similarities between PYP and rhodopsins (Rh) are discussed.

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Ultrafast photoinduced reaction dynamics of photoactive yellow protein (PYP): Observation of coherent oscillations in the femtosecond fluorescence decay dynamics

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