Ultrafast hydrogen-bonding dynamics in the electronic excited state of photoactive yellow protein revealed by femtosecond stimulated raman spectroscopy

Ryosuke Nakamura, Norio Hamada, Kenta Abe, Masayuki Yoshizawa

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

The ultrafast structural dynamics in the electronic excited state of photoactive yellow protein (PYP) is studied by femtosecond stimulated Raman spectroscopy. Stimulated Raman spectra in the electronic excited state, S 1, can be obtained by using a Raman pump pulse in resonance with the S1-S0 transition. This is confirmed by comparing the experimental results with numerical calculations based on the density matrix treatment. We also investigate the hydrogen-bonding network surrounding the wild-type (WT)-PYP chromophore in the ground and excited states by comparing its stimulated Raman spectra with those of the E46Q-PYP mutant. We focus on the relative intensity of the Raman band at 1555 cm-1, which includes both vinyl bond Cî - C stretching and ring vibrations and is sensitive to the hydrogen-bonding network around the phenolic oxygen of the chromophore. The relative intensity for the WT-PYP decreases after actinic excitation within the 150 fs time resolution and reaches a similar intensity to that for E46Q-PYP. These observations indicate that the WT-PYP hydrogen-bonding network is immediately rearranged in the electronic excited state to form a structure similar to that of E46Q-PYP.

Original languageEnglish
Pages (from-to)14768-14775
Number of pages8
JournalJournal of Physical Chemistry B
Volume116
Issue number51
DOIs
Publication statusPublished - 2012 Dec 27

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

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