Ubiquitin-conjugating enzyme Cdc34 mediates methylmercury resistance in Saccharomyces cerevisiae by increasing Whi2 degradation

Gi Wook Hwang, Yousuke Ogiwara, Tsutomu Takahashi, Akira Naganuma

Research output: Contribution to journalLetterpeer-review

4 Citations (Scopus)

Abstract

Overexpression of Cdc34, a ubiquitin-conjugating enzyme, confers methylmercury resistance on yeast cells. This suggests that degradation, by the ubiquitin-proteasome (UP) system, of proteins that enhance methylmercury toxicity might be a factor in the development of methylmercury resistance. The present study shows that yeast cells overexpressing Whi2, a protein that is ubiquitinated in cells, are highly susceptible to methylmercury, suggesting that Whi2 may enhance methylmercury toxicity. Although both Whi2 deficiency and Cdc34 overexpression individually confer methylmercury resistance on yeast cells, Whi2-deficient cells overexpressing Cdc34 showed no additive resistance to methylmercury, compared with Whi2-expressing cells that overexpress Cdc34. The intracellular level of Whi2 was significantly reduced by Cdc34 overexpression; however, this reduction was almost completely attenuated when proteasomal degradation was inhibited. These results suggest that overexpression of Cdc34 confers methylmercury resistance on yeast cells through the UP system by promoting degradation of Whi2, a methylmercury toxicity-enhancing protein.

Original languageEnglish
Pages (from-to)1283-1286
Number of pages4
JournalJournal of Toxicological Sciences
Volume37
Issue number6
DOIs
Publication statusPublished - 2012

Keywords

  • Cdc34
  • Methylmercury
  • Ubiquitination
  • Whi2
  • Yeast

ASJC Scopus subject areas

  • Toxicology

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