Tyrosine phosphorylation of phospholipase Cγ in c-met/HGF receptor-stimulated hepatocytes: Comparison with HepG2 hepatocarcinoma cells

Yukio Okano, Kensaku Mizuno, Sinji Osada, Toshikazu Nakamura, Yoshinori Nozawa

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)

Abstract

Hepatocyte growth factor (HGF) stimulates inositol 1,4,5-trisphosphate (InsP3) formation in rat primary cultured hepatocytes, which is inhibited by the pretreatment with a tyrosine kinase inhibitor, genistein. This lnsP3 production was coincident with tyrosine phosphorylation of phospholipase Cγ (PLCγ), detected in immunoprecipitates with anti-PLCγ, suggesting activation mechanism of PLCγ by tyrosine phosphorylation. However, in human hepatocarcinoma HepG2 cells, HGF, which suppresses cell growth, causes neither phosphorylation of PLCγ nor lnsP3 formation. The results suggests that PLCγ in normal hepatocytes was activated by HGF through tyrosine kinase of HGF receptor.

Original languageEnglish
Pages (from-to)842-848
Number of pages7
JournalBiochemical and biophysical research communications
Volume190
Issue number3
DOIs
Publication statusPublished - 1993 Feb 15
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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