Type III secretion system of bradyrhizobium sp. Sutn9-2 obstructs symbiosis with lotus spp.

Shun Hashimoto; Kohki Goto; Pongdet Pyromyou; Pongpan Songwattana; Teerana Greetatorn; Panlada Tittabutr; Nantakorn Boonkerd; Neung Teaumroong; Toshiki Uchiumi

doi:10.1264/jsme2.ME20041

Type III secretion system of bradyrhizobium sp. Sutn9-2 obstructs symbiosis with lotus spp.

Shun Hashimoto, Kohki Goto, Pongdet Pyromyou, Pongpan Songwattana, Teerana Greetatorn, Panlada Tittabutr, Nantakorn Boonkerd, Neung Teaumroong, Toshiki Uchiumi

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The rhizobial type III secretion system secretes effector proteins into host plant cells, which may either promote or inhibit symbiosis with legumes. We herein demonstrated that the type III secretion system of Bradyrhizobium sp. SUTN9-2 obstructed symbiosis with Lotus japonicus Miyakojima, L. japonicus Gifu, and Lotus burttii. A mutant of SUTN9-2 that is unable to secrete effector proteins showed better nodulation and plant growth promotion than wild-type SUTN9-2 when paired with these Lotus spp. We propose that SUTN9-2 is a useful strain for understanding the mechanisms by which effector proteins obstruct symbiosis between Bradyrhizobium and Lotus spp.

Original language	English
Article number	ME20041
Pages (from-to)	1-5
Number of pages	5
Journal	Microbes and environments
Volume	35
Issue number	3
DOIs	https://doi.org/10.1264/jsme2.ME20041
Publication status	Published - 2020
Externally published	Yes

Keywords

Bradyrhizobium
Effector protein
Lotus spp
Symbiosis
Type III secretion system

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Soil Science
Plant Science

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10.1264/jsme2.ME20041

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title = "Type III secretion system of bradyrhizobium sp. Sutn9-2 obstructs symbiosis with lotus spp.",

abstract = "The rhizobial type III secretion system secretes effector proteins into host plant cells, which may either promote or inhibit symbiosis with legumes. We herein demonstrated that the type III secretion system of Bradyrhizobium sp. SUTN9-2 obstructed symbiosis with Lotus japonicus Miyakojima, L. japonicus Gifu, and Lotus burttii. A mutant of SUTN9-2 that is unable to secrete effector proteins showed better nodulation and plant growth promotion than wild-type SUTN9-2 when paired with these Lotus spp. We propose that SUTN9-2 is a useful strain for understanding the mechanisms by which effector proteins obstruct symbiosis between Bradyrhizobium and Lotus spp.",

keywords = "Bradyrhizobium, Effector protein, Lotus spp, Symbiosis, Type III secretion system",

author = "Shun Hashimoto and Kohki Goto and Pongdet Pyromyou and Pongpan Songwattana and Teerana Greetatorn and Panlada Tittabutr and Nantakorn Boonkerd and Neung Teaumroong and Toshiki Uchiumi",

note = "Funding Information: We are especially grateful to Prof. Shusei Sato and Dr. Shohei Kusakabe (Tohoku University, Japan) for providing their data related to Bradyrhizobium elkanii USDA61. We thank the National BioResource Project for providing seeds of Lotus japonicus Miya-kojima and Gifu and Lotus burttii. We also thank Ms. Yukino Yoshimine (Kagoshima University, Japan) for her excellent technical assistance. This work was partially supported by the National Institute for Basic Biology (NIBB) Collaborative Research Program No. 19-353 and No. 20-314, and by Suranaree University of Technology. Publisher Copyright: {\textcopyright} 2020, Japanese Society of Microbial Ecology. All rights reserved.",

year = "2020",

doi = "10.1264/jsme2.ME20041",

language = "English",

volume = "35",

pages = "1--5",

journal = "Microbes and Environments",

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publisher = "Japanese Society Of Microbial Ecology",

number = "3",

}

TY - JOUR

T1 - Type III secretion system of bradyrhizobium sp. Sutn9-2 obstructs symbiosis with lotus spp.

AU - Hashimoto, Shun

AU - Goto, Kohki

AU - Pyromyou, Pongdet

AU - Songwattana, Pongpan

AU - Greetatorn, Teerana

AU - Tittabutr, Panlada

AU - Boonkerd, Nantakorn

AU - Teaumroong, Neung

AU - Uchiumi, Toshiki

N1 - Funding Information: We are especially grateful to Prof. Shusei Sato and Dr. Shohei Kusakabe (Tohoku University, Japan) for providing their data related to Bradyrhizobium elkanii USDA61. We thank the National BioResource Project for providing seeds of Lotus japonicus Miya-kojima and Gifu and Lotus burttii. We also thank Ms. Yukino Yoshimine (Kagoshima University, Japan) for her excellent technical assistance. This work was partially supported by the National Institute for Basic Biology (NIBB) Collaborative Research Program No. 19-353 and No. 20-314, and by Suranaree University of Technology. Publisher Copyright: © 2020, Japanese Society of Microbial Ecology. All rights reserved.

PY - 2020

Y1 - 2020

N2 - The rhizobial type III secretion system secretes effector proteins into host plant cells, which may either promote or inhibit symbiosis with legumes. We herein demonstrated that the type III secretion system of Bradyrhizobium sp. SUTN9-2 obstructed symbiosis with Lotus japonicus Miyakojima, L. japonicus Gifu, and Lotus burttii. A mutant of SUTN9-2 that is unable to secrete effector proteins showed better nodulation and plant growth promotion than wild-type SUTN9-2 when paired with these Lotus spp. We propose that SUTN9-2 is a useful strain for understanding the mechanisms by which effector proteins obstruct symbiosis between Bradyrhizobium and Lotus spp.

AB - The rhizobial type III secretion system secretes effector proteins into host plant cells, which may either promote or inhibit symbiosis with legumes. We herein demonstrated that the type III secretion system of Bradyrhizobium sp. SUTN9-2 obstructed symbiosis with Lotus japonicus Miyakojima, L. japonicus Gifu, and Lotus burttii. A mutant of SUTN9-2 that is unable to secrete effector proteins showed better nodulation and plant growth promotion than wild-type SUTN9-2 when paired with these Lotus spp. We propose that SUTN9-2 is a useful strain for understanding the mechanisms by which effector proteins obstruct symbiosis between Bradyrhizobium and Lotus spp.

KW - Bradyrhizobium

KW - Effector protein

KW - Lotus spp

KW - Symbiosis

KW - Type III secretion system

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Type III secretion system of bradyrhizobium sp. Sutn9-2 obstructs symbiosis with lotus spp.

Abstract

Keywords

ASJC Scopus subject areas

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