Type II alveolar epithelial cells in lung express receptor for advanced glycation end products (RAGE) gene

Fumiki Katsuoka, Yasushi Kawakami, Takeo Arai, Hiroyuki Imuta, Masachika Fujiwara, Hiroshi Kanma, Kamejiro Yamashita

Research output: Contribution to journalArticlepeer-review

67 Citations (Scopus)

Abstract

Messenger RNA of receptor for advanced glycation end products (RAGE) is abundantly expressed in the lung. However, cell types expressing RAGE mRNA in the lung have not been identified. In order to elucidate the function of RAGE in pulmonary tissue, we have identified a cell type expressing RAGE mRNA by in situ hybridization and compared its expression level of RAGE mRNA by RNA blot analysis of isolated cells. In situ hybridization revealed that RAGE mRNA was intensely and specifically visualized in alveolar epithelial type II (AT-II) cells, and weakly in alveolar macrophages. The expression of RAGE mRNA in the primary culture of AT-II cells was at a high level, but that in alveolar macrophages isolated from alveolar lavage was under the level of detection by RNA blot analysis. These results showed that RAGE mRNA is specifically expressed in AT-II cells, and suggested that RAGE makes a substantial contribution to the function of AT-II cells in the lung.

Original languageEnglish
Pages (from-to)512-516
Number of pages5
JournalBiochemical and biophysical research communications
Volume238
Issue number2
DOIs
Publication statusPublished - 1997 Sep 18
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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