Type 1 and type 2 human PrPSc have different aggregation sizes in methionine homozygotes with sporadic, iatrogenic and variant Creutzfeldt-Jakob disease

Atsushi Kobayashi, Sakae Satoh, James W. Ironside, Shirou Mohri, Tetsuyuki Kitamoto

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In Creutzfeldt-Jakob disease (CJD), the type (type 1 or 2) of abnormal isoform of the prion protein (PrPSc) in the brain and the genotype at codon 129 of the PrP gene are major determinants of clinicopathological phenotype. Little is known about the difference in biochemical properties between the two types of PrPSc, except for the different proteinase K cleavage sites. To investigate the size of aggregates formed by PrPSc types 1 and 2, brain homogenates from various cases of CJD with the same genotype (homozygous for methionine at codon 129) were passed through filters with a mean pore size of 72±4 nm. Type 2 PrPSc was efficiently removed from the filtrates by the filters, in contrast to type 1. Even type 2 PrPSc from a patient without amyloid plaques was removed more efficiently than type 1 from patients with amyloid plaques. These results indicate that type 2 PrPSc has a larger aggregation size than type 1, irrespective of the existence of amyloid plaques.

Original languageEnglish
Pages (from-to)237-240
Number of pages4
JournalJournal of General Virology
Volume86
Issue number1
DOIs
Publication statusPublished - 2005 Jan

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Type 1 and type 2 human PrP<sup>Sc</sup> have different aggregation sizes in methionine homozygotes with sporadic, iatrogenic and variant Creutzfeldt-Jakob disease'. Together they form a unique fingerprint.

  • Cite this