Two variable lymphocyte receptor genes of the inshore hagfish are located far apart on the same chromosome

Jun Kasamatsu, Takashi Suzuki, Junko Ishijima, Yoichi Matsuda, Masanori Kasahara

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Variable lymphocyte receptors (VLR) generate enormous diversity through assembling highly diverse leucine-rich repeat (LRR) modules and presumably function as antigen receptors in jawless vertebrates. The hagfish, which constitute major extant members of jawless vertebrates along with lampreys, have two VLR genes designated VLRA and VLRB, whereas only a single VLR gene has been identified in the lamprey. In the present study, we show by fluorescence in situ hybridization (FISH) that hagfish VLRA and VLRB are located on the same chromosome, but are far apart from each other. Analysis of available inshore hagfish complementary DNA sequences indicates that VLRA and VLRB do not share a LRR module with an identical nucleotide sequence. Physical separation of VLRA and VLRB is consistent with this observation and indicates that the two VLR genes function as separate units. The FISH protocol developed in this study should be useful for the analysis of the agnathan genome.

Original languageEnglish
Pages (from-to)329-331
Number of pages3
JournalImmunogenetics
Volume59
Issue number4
DOIs
Publication statusPublished - 2007 Apr
Externally publishedYes

Keywords

  • Fluorescence in situ hybridization
  • Hagfish
  • Jawless vertebrates
  • LRR
  • VLR

ASJC Scopus subject areas

  • Immunology
  • Genetics

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