Two-photon STED nanoscopy realizing 100-nm spatial resolution utilizing high-peak-power sub-nanosecond 655-nm pulses

Hirokazu Ishii, Kohei Otomo, Jui Hung Hung, Motosuke Tsutsumi, Hiroyuki Yokoyama, Tomomi Nemoto

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

We developed two-photon excitation stimulated emission depletion (STED) nanoscopy using high-peak-power sub-nanosecond 655-nm pulses. The STED pulse exhibited ideal optical properties and sufficient pulse energy to realize a 70-nm spatial resolution in the compact setup with electrically controllable components. For biological applications, we screened suitable fluorescent dyes or proteins and realized the sub-100 nm spatial resolution imaging of presynaptic protein clusters in fixed primary cultured neurons without severe photobleaching. We expect this method to enable visualization of ultrastructures and the cluster dynamics of biomolecules representing physiological functions in living cells and tissue.

Original languageEnglish
Article number360910
Pages (from-to)3104-3113
Number of pages10
JournalBiomedical Optics Express
Volume10
Issue number7
DOIs
Publication statusPublished - 2019 Jul

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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