Two-photon fluorescence imaging with a pulse source based on a 980-nm gain-switched laser diode

Kenji Taira, Takeshi Hashimoto, Hiroyuki Yokoyama

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

We developed a compact, high-peak-power picosecond pulse source based on a gain-switched laser diode with an emission wavelength of 980 nm and successfully demonstrated two-photon fluorescence imaging. The pulse source consisted of the gain-switched laser diode, a pulse compressor, a pulse reshaper, and optical amplifiers, all connected by single-mode fibers. The optical pulse generated by the pulse source had a pulse width of 3.5 ps, an average power of 35 mW, and kilowatt-level peak power at a repetition rate of 10 MHz. Alexa488-stained Glomeruli and convoluted tubules of a mouse kidney section were observed by two-photon imaging with the developed pulse source.

Original languageEnglish
Pages (from-to)2454-2458
Number of pages5
JournalOptics Express
Volume15
Issue number5
DOIs
Publication statusPublished - 2007 Mar 5

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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