Two-photon fluorescence bioimaging with an all-semiconductor laser picosecond pulse source

Masaru Kuramoto, Nobuyoshi Kitajima, Hengchang Guo, Yuji Furushima, Masao Ikeda, Hiroyuki Yokoyama

Research output: Contribution to journalArticlepeer-review

76 Citations (Scopus)

Abstract

We have demonstrated successful two-photon excitation fluorescence bioimaging using a high-power pulsed all-semiconductor laser. Toward this purpose, we developed a pulsed light source consisting of a mode-locked laser diode and a two-stage diode laser amplifier. This pulsed light source provided optical pulses of 5 ps duration and having a maximum peak power of over 100 W at a wavelength of 800 nm and a repetition frequency of 500 MHz.

Original languageEnglish
Pages (from-to)2726-2728
Number of pages3
JournalOptics Letters
Volume32
Issue number18
DOIs
Publication statusPublished - 2007 Sep 15

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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