Two-photon excitation STED microscopy by utilizing transmissive liquid crystal devices

Kohei Otomo, Terumasa Hibi, Yuichi Kozawa, Makoto Kurihara, Nobuyuki Hashimoto, Hiroyuki Yokoyama, Shunichi Sato, Tomomi Nemoto

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


Transmissive liquid crystal devices (tLCDs) enable the modification of optical properties, such as phase, polarization, and laser light intensity, over a wide wavelength region at a high conversion efficiency. By utilizing tLCDs, we developed a new two-photon excitation stimulated emission depletion microscopy technique based on a conventional two-photon microscope. Spatial resolution was improved by compensating for phase shifts distributed in the optical path. Using this technique, we observed the fine structures of microtubule networks in fixed biological cells.

Original languageEnglish
Pages (from-to)28215-28221
Number of pages7
JournalOptics Express
Issue number23
Publication statusPublished - 2014 Nov 17

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics


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