TY - JOUR
T1 - Two-photon excitation imaging of exocytosis and endocytosis and determination of their spatial organization
AU - Kasai, Haruo
AU - Kishimoto, Takuya
AU - Nemoto, Tomomi
AU - Hatakeyama, Hiroyasu
AU - Liu, Ting Ting
AU - Takahashi, Noriko
PY - 2006/10/31
Y1 - 2006/10/31
N2 - Two-photon excitation imaging is the least invasive optical approach to study living tissues. We have established two-photon extracellular polar-tracer (TEP) imaging with which it is possible to visualize and quantify all exocytic events in the plane of focus within secretory tissues. This technology also enables estimate of the precise diameters of vesicles independently of the spatial resolution of the optical microscope, and determination of the fusion pore dynamics at nanometer resolution using TEP-imaging based quantification (TEPIQ). TEP imaging has been applied to representative secretory glands, e.g., exocrine pancreas, endocrine pancreas, adrenal medulla and a pheochromocytoma cell line (PC12), and has revealed unexpected diversity in the spatial organization of exocytosis and endocytosis crucial for the physiology and pathology of secretory tissues and neurons. TEP imaging and TEPIQ analysis are powerful tools for elucidating the molecular and cellular mechanisms of exocytosis and certain related diseases, such as diabetes mellitus, and the development of new therapeutic agents and diagnostic tools.
AB - Two-photon excitation imaging is the least invasive optical approach to study living tissues. We have established two-photon extracellular polar-tracer (TEP) imaging with which it is possible to visualize and quantify all exocytic events in the plane of focus within secretory tissues. This technology also enables estimate of the precise diameters of vesicles independently of the spatial resolution of the optical microscope, and determination of the fusion pore dynamics at nanometer resolution using TEP-imaging based quantification (TEPIQ). TEP imaging has been applied to representative secretory glands, e.g., exocrine pancreas, endocrine pancreas, adrenal medulla and a pheochromocytoma cell line (PC12), and has revealed unexpected diversity in the spatial organization of exocytosis and endocytosis crucial for the physiology and pathology of secretory tissues and neurons. TEP imaging and TEPIQ analysis are powerful tools for elucidating the molecular and cellular mechanisms of exocytosis and certain related diseases, such as diabetes mellitus, and the development of new therapeutic agents and diagnostic tools.
KW - Adrenal medulla
KW - Chromaffin cell
KW - Compound exocytosis
KW - Exocrine gland
KW - Insulin secretion
KW - Pancreatic islet
KW - Pheochromocytoma cell
KW - Synapse
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U2 - 10.1016/j.addr.2006.07.008
DO - 10.1016/j.addr.2006.07.008
M3 - Review article
C2 - 16996640
AN - SCOPUS:33750720622
VL - 58
SP - 850
EP - 877
JO - Advanced Drug Delivery Reviews
JF - Advanced Drug Delivery Reviews
SN - 0169-409X
IS - 7
ER -
