Two-Photon calcium imaging of the medial prefrontal cortex and hippocampus without cortical invasion

Masashi Kondo, Kenta Kobayashi, Masamichi Ohkura, Junichi Nakai, Masanori Matsuzaki

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

In vivo two-photon calcium imaging currently allows us to observe the activity of multiple neurons up to ~900 mm below the cortical surface without cortical invasion. However, many important brain areas are located deeper than this. Here, we used an 1100 nm laser that underfilled the back aperture of the objective together with red genetically encoded calcium indicators to establish two-photon calcium imaging of the intact mouse brain and detect neural activity up to 1200 mm from the cortical surface. This imaging was obtained from the medial prefrontal cortex (the prelimbic area) and the hippocampal CA1 region. We found that neural activity before water delivery repeated at a constant interval was higher in the prelimbic area than in layer 2/3 of the secondary motor area. Reducing the invasiveness of imaging is an important strategy to reveal the intact brain processes active in cognition and memory.

Original languageEnglish
Article numbere26839
JournaleLife
Volume6
DOIs
Publication statusPublished - 2017 Sep 25
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Two-Photon calcium imaging of the medial prefrontal cortex and hippocampus without cortical invasion'. Together they form a unique fingerprint.

Cite this