Two-photon calcium imaging of medial prefrontal cortex and hippocampus without cortical invasion

Masashi Kondo, Kenta Kobayashi, Masamichi Ohkura, Junichi Nakai, Masanori Matsuzaki

Research output: Contribution to journalArticlepeer-review


In vivo two-photon calcium imaging currently allows us to observe the activity of multiple neurons up to ∼900 μm below the cortical surface without cortical invasion. However, many other important brain areas are located deeper than this. Here, we used a 1100 nm laser, which underfilled the back aperture of the objective, and red genetically encoded calcium indicators to establish two-photon calcium imaging of the intact mouse brain and detect neural activity up to 1200 μm from the cortical surface. This imaging was obtained from the medial prefrontal cortex (the prelimbic area) and the hippocampal CA1 region. We found that the neural activity related to reward prediction is higher in the prelimbic area than in layer 2/3 of the secondary motor area, while it is negligible in the hippocampal CA1 region. Reducing the invasiveness of imaging is an important strategy to reveal the brain processes active in cognition and memory.

Original languageEnglish
JournalUnknown Journal
Publication statusPublished - 2017 Mar 22

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Fingerprint Dive into the research topics of 'Two-photon calcium imaging of medial prefrontal cortex and hippocampus without cortical invasion'. Together they form a unique fingerprint.

Cite this