Two-photon bioimaging with picosecond optical pulses from a semiconductor laser

Hiroyuki Yokoyama, Hengchang Guo, Takuya Yoda, Keijiro Takashima, Ki Ichi Sato, Hirokazu Taniguchi, Hiromasa Ito

Research output: Contribution to journalArticlepeer-review

62 Citations (Scopus)

Abstract

Toward a practical light source for two-photon bioimaging, we have generated kilowatt peak power of 0.77 μm wavelength and 5 ps optical pulse via second-harmonic generation of the amplified output from a gain-switched 1.55 μm semiconductor laser. This compact scheme and stable optical-pulse-source has been successfully used for the two-photon fluorescence bioimaging of actin filaments in PtK2 cells.

Original languageEnglish
Pages (from-to)3467-3471
Number of pages5
JournalOptics Express
Volume14
Issue number8
DOIs
Publication statusPublished - 2006 Apr 17

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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