TY - GEN
T1 - Two-photon bioimaging with a multi-kilowatt peak-power optical pulse source using a 1-μm wavelength mode-locked semiconductor laser
AU - Guo, Hengchang
AU - Takashima, Keijiro
AU - Sato, Aya
AU - Yokoyama, Hiroyuki
AU - Mure, Masahito
AU - Iseki, Yuji
AU - Tsubokawa, Hiroshi
PY - 2008
Y1 - 2008
N2 - We have developed a multi-kilowatt peak power 1-um optical pulse source for two-photon microscopy. Utilizing an external-cavity hybrid mode-locked semiconductor laser, we were able to generate picosecond optical pulses at a 500-MHz repetition rate. With a semiconductor optical amplifier driven by synchronized electronic gating pulses, the optical pulse repetition rate was sub-harmonically extracted at 1-100 MHz. At a 10-MHz repetition rate, optical pulses were then amplified to a peak power of greater than 2 kW with a two-stage Yb-doped fiber amplifier. Using this light source, we successfully obtained clear two-photon images of mouse brain neurons expressing green fluorescent proteins.
AB - We have developed a multi-kilowatt peak power 1-um optical pulse source for two-photon microscopy. Utilizing an external-cavity hybrid mode-locked semiconductor laser, we were able to generate picosecond optical pulses at a 500-MHz repetition rate. With a semiconductor optical amplifier driven by synchronized electronic gating pulses, the optical pulse repetition rate was sub-harmonically extracted at 1-100 MHz. At a 10-MHz repetition rate, optical pulses were then amplified to a peak power of greater than 2 kW with a two-stage Yb-doped fiber amplifier. Using this light source, we successfully obtained clear two-photon images of mouse brain neurons expressing green fluorescent proteins.
KW - Fiber amplifiers
KW - Fluorescence microscopy
KW - Fluorescent proteins
KW - Medical and biological imaging
KW - Multiphoton processes
KW - Picosecond phenomena
KW - Semiconductor lasers
UR - http://www.scopus.com/inward/record.url?scp=42149188209&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=42149188209&partnerID=8YFLogxK
U2 - 10.1117/12.762922
DO - 10.1117/12.762922
M3 - Conference contribution
AN - SCOPUS:42149188209
SN - 9780819470355
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Multiphoton Microscopy in the Biomedical Sciences VIII
T2 - Multiphoton Microscopy in the Biomedical Sciences VIII
Y2 - 20 January 2008 through 22 January 2008
ER -