Two-photon bioimaging with a multi-kilowatt peak-power optical pulse source using a 1-μm wavelength mode-locked semiconductor laser

Hengchang Guo; Keijiro Takashima; Aya Sato; Hiroyuki Yokoyama; Masahito Mure; Yuji Iseki; Hiroshi Tsubokawa

doi:10.1117/12.762922

Two-photon bioimaging with a multi-kilowatt peak-power optical pulse source using a 1-μm wavelength mode-locked semiconductor laser

Hengchang Guo, Keijiro Takashima, Aya Sato, Hiroyuki Yokoyama, Masahito Mure, Yuji Iseki, Hiroshi Tsubokawa

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

4 Citations (Scopus)

Abstract

We have developed a multi-kilowatt peak power 1-um optical pulse source for two-photon microscopy. Utilizing an external-cavity hybrid mode-locked semiconductor laser, we were able to generate picosecond optical pulses at a 500-MHz repetition rate. With a semiconductor optical amplifier driven by synchronized electronic gating pulses, the optical pulse repetition rate was sub-harmonically extracted at 1-100 MHz. At a 10-MHz repetition rate, optical pulses were then amplified to a peak power of greater than 2 kW with a two-stage Yb-doped fiber amplifier. Using this light source, we successfully obtained clear two-photon images of mouse brain neurons expressing green fluorescent proteins.

Original language	English
Title of host publication	Multiphoton Microscopy in the Biomedical Sciences VIII
DOIs	https://doi.org/10.1117/12.762922
Publication status	Published - 2008
Event	Multiphoton Microscopy in the Biomedical Sciences VIII - San Jose, CA, United States Duration: 2008 Jan 20 → 2008 Jan 22

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	6860
ISSN (Print)	1605-7422

Other

Other	Multiphoton Microscopy in the Biomedical Sciences VIII
Country/Territory	United States
City	San Jose, CA
Period	08/1/20 → 08/1/22

Keywords

Fiber amplifiers
Fluorescence microscopy
Fluorescent proteins
Medical and biological imaging
Multiphoton processes
Picosecond phenomena
Semiconductor lasers

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.762922

Cite this

Guo, H., Takashima, K., Sato, A., Yokoyama, H., Mure, M., Iseki, Y., & Tsubokawa, H. (2008). Two-photon bioimaging with a multi-kilowatt peak-power optical pulse source using a 1-μm wavelength mode-locked semiconductor laser. In Multiphoton Microscopy in the Biomedical Sciences VIII [686020] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6860). https://doi.org/10.1117/12.762922

Two-photon bioimaging with a multi-kilowatt peak-power optical pulse source using a 1-μm wavelength mode-locked semiconductor laser. / Guo, Hengchang; Takashima, Keijiro; Sato, Aya et al.

Multiphoton Microscopy in the Biomedical Sciences VIII. 2008. 686020 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6860).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Guo, H, Takashima, K, Sato, A, Yokoyama, H, Mure, M, Iseki, Y & Tsubokawa, H 2008, Two-photon bioimaging with a multi-kilowatt peak-power optical pulse source using a 1-μm wavelength mode-locked semiconductor laser. in Multiphoton Microscopy in the Biomedical Sciences VIII., 686020, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, San Jose, CA, United States, 08/1/20. https://doi.org/10.1117/12.762922

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Two-photon bioimaging with a multi-kilowatt peak-power optical pulse source using a 1-μm wavelength mode-locked semiconductor laser

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