Two-photon bioimaging with a multi-kilowatt peak-power optical pulse source using a 1-μm wavelength mode-locked semiconductor laser

Hengchang Guo, Keijiro Takashima, Aya Sato, Hiroyuki Yokoyama, Masahito Mure, Yuji Iseki, Hiroshi Tsubokawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

We have developed a multi-kilowatt peak power 1-um optical pulse source for two-photon microscopy. Utilizing an external-cavity hybrid mode-locked semiconductor laser, we were able to generate picosecond optical pulses at a 500-MHz repetition rate. With a semiconductor optical amplifier driven by synchronized electronic gating pulses, the optical pulse repetition rate was sub-harmonically extracted at 1-100 MHz. At a 10-MHz repetition rate, optical pulses were then amplified to a peak power of greater than 2 kW with a two-stage Yb-doped fiber amplifier. Using this light source, we successfully obtained clear two-photon images of mouse brain neurons expressing green fluorescent proteins.

Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences VIII
DOIs
Publication statusPublished - 2008 Apr 21
EventMultiphoton Microscopy in the Biomedical Sciences VIII - San Jose, CA, United States
Duration: 2008 Jan 202008 Jan 22

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6860
ISSN (Print)1605-7422

Other

OtherMultiphoton Microscopy in the Biomedical Sciences VIII
CountryUnited States
CitySan Jose, CA
Period08/1/2008/1/22

Keywords

  • Fiber amplifiers
  • Fluorescence microscopy
  • Fluorescent proteins
  • Medical and biological imaging
  • Multiphoton processes
  • Picosecond phenomena
  • Semiconductor lasers

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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